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The RNA-binding protein Musashi2 regulates asymmetric neural precursor cell divisions of the developing cerebral cortex

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dc.contributor.advisor Vessey, John P.
dc.contributor.author Reynolds, Kathryn E.
dc.date.accessioned 2016-08-25T13:28:23Z
dc.date.available 2017-08-19T05:00:13Z
dc.date.copyright 2016-08
dc.date.created 2016-08-19
dc.date.issued 2016-08-25
dc.identifier.uri http://hdl.handle.net/10214/9884
dc.description.abstract The brain’s complex cortical circuitry is generated through asymmetric divisions of neural precursor cells (NPCs). These divisions produce one NPC plus one differentiated neuron or intermediate progenitor, due in part to localization of fate determinants to a single region of the dividing cell. This research identifies Musashi2 (mMsi2) as a critical component of cortical asymmetric NPC division. Temporal expression profiles and in vitro immunofluorescence detected mMsi2 within NPCs and some neurons of the mouse cortex throughout neurogenesis. shRNA knockdown in cortical primary culture demonstrated that mMsi2 loss increases NPCs and proportionally decreases neurons and intermediate progenitors, while mMsi2 knockdown in utero similarly increased cells within immature layers of the mouse cortex. mMsi2 overexpression in cortical primary culture again increased NPCs, suggesting that developmental mMsi2 expression must be tightly regulated. Together, these results indicate that mMsi2 promotes neuronal formation during asymmetric NPC division, and is therefore essential to cortical development. en_US
dc.description.sponsorship NSERC; Scottish Rite Charitable Foundation en_US
dc.language.iso en en_US
dc.rights Attribution-NonCommercial-NoDerivs 2.5 Canada *
dc.rights.uri http://creativecommons.org/licenses/by-nc-nd/2.5/ca/ *
dc.subject embryonic brain development en_US
dc.subject asymmetric cell division en_US
dc.subject RNA-binding protein en_US
dc.subject mRNA localization en_US
dc.subject mouse model en_US
dc.subject neural stem cell en_US
dc.subject Musashi2 en_US
dc.title The RNA-binding protein Musashi2 regulates asymmetric neural precursor cell divisions of the developing cerebral cortex en_US
dc.type Thesis en_US
dc.degree.programme Molecular and Cellular Biology en_US
dc.degree.name Master of Science en_US
dc.degree.department Department of Molecular and Cellular Biology en_US


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Attribution-NonCommercial-NoDerivs 2.5 Canada Except where otherwise noted, this item's license is described as Attribution-NonCommercial-NoDerivs 2.5 Canada