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The Impacts of ProP Putative Interactors on the Localization and Activity of the Transporter ProP, and on Escherichia coli Phenotype

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dc.contributor.advisor Wood, Janet
dc.contributor.author Gonzalez, Karen
dc.date.accessioned 2016-06-28T17:32:59Z
dc.date.available 2016-06-28T17:32:59Z
dc.date.copyright 2016-07
dc.date.created 2016-06-22
dc.date.issued 2016-06-28
dc.identifier.uri http://hdl.handle.net/10214/9771
dc.description.abstract Understanding the mechanism by which ProP (an osmosensing transporter in Escherichia coli) localizes to the poles of cells might uncover a valuable model of how protein function might relate to protein organization in bacteria. Evidence pointed to involvement of a ProP-protein interaction in its observed localization pattern. To further study if ProP protein interactors contribute to this phenomenon, ProP was tagged with a fluorescent protein, thereby supplementing the chemical labeling method previously used for visualization. Though an attempt was made to improve fusion protein stability, herein we show that prevalent degradation has altered the location of tagged ProP within cells. YdhP and YjbJ were identified as putative ProP interactors and were chosen for further study. Deletion of yjbJ did not alter culture growth or cell morphology. Elimination of YdhP or YjbJ expression did not affect ProP activity or localization, thus impact of these ProP-protein interactions was not evident. en_US
dc.description.sponsorship NSERC en_US
dc.language.iso en en_US
dc.rights Attribution-NonCommercial-NoDerivs 2.5 Canada *
dc.rights.uri http://creativecommons.org/licenses/by-nc-nd/2.5/ca/ *
dc.subject Microbiology en_US
dc.subject E. coli en_US
dc.subject Microscopy en_US
dc.subject GFP en_US
dc.subject ProP en_US
dc.subject transporter en_US
dc.subject FlAsH-EDT2 en_US
dc.subject morphology en_US
dc.subject growth en_US
dc.subject fluorescence en_US
dc.subject localization en_US
dc.subject imaging en_US
dc.subject fusion protein en_US
dc.subject osmoregulation en_US
dc.subject osmotic en_US
dc.subject interacting protein en_US
dc.subject YdhP en_US
dc.subject YjbJ en_US
dc.subject BACTH en_US
dc.subject cardiolipin en_US
dc.subject membrane en_US
dc.subject bacterial en_US
dc.title The Impacts of ProP Putative Interactors on the Localization and Activity of the Transporter ProP, and on Escherichia coli Phenotype en_US
dc.type Thesis en_US
dc.degree.programme Molecular and Cellular Biology en_US
dc.degree.name Master of Science en_US
dc.degree.department Department of Molecular and Cellular Biology en_US
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Attribution-NonCommercial-NoDerivs 2.5 Canada Except where otherwise noted, this item's license is described as Attribution-NonCommercial-NoDerivs 2.5 Canada