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Protein Domains of Vitis CBF1 and Translational Regulation of Cold-Induced Genes via Codon Usage

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dc.contributor.advisor Annette, Nassuth Faultless, JW Trent 2016-05-13T12:49:06Z 2016-05-13T12:49:06Z 2016-05 2016-04-07 2016-05-13
dc.description.abstract CBF (C-repeat Binding Factor) transcription factors are vital in ABA-independent signalling during cold acclimation and subsequent freezing tolerance in Vitis. We investigated nuclear localization signals within CBF1; known to be rapidly expressed following exposure to cold; as well as potential alternative translation products of CBF1 stemming from stop codon readthrough. As a result of our interest in suppressor tRNA species and their potential influence on alternative translation products of CBF1, and due to recent developments concerning codon use of genes which demonstrate tissue-specific expression in Vitis, we also examined codon use of genes involved in cold acclimation and freezing tolerance. We demonstrate roles in both activation and localization for two domains of CBF1, while previously each domain was ascribed a single role. We additionally demonstrate that cold regulated genes share a distinct pattern of codon use in Vitis. en_US
dc.description.sponsorship NSERC, OGS, Grape Grower's of Ontario en_US
dc.language.iso en en_US
dc.subject CBF en_US
dc.subject Grape en_US
dc.subject Codon use en_US
dc.subject stress en_US
dc.subject cold en_US
dc.subject NLS en_US
dc.title Protein Domains of Vitis CBF1 and Translational Regulation of Cold-Induced Genes via Codon Usage en_US
dc.type Thesis en_US Molecular and Cellular Biology en_US Master of Science en_US Department of Molecular and Cellular Biology en_US
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