Main content

A new group of eubacterial light-driven retinal-binding proton pumps with an unusual cytoplasmic proton donor

Show full item record

Title: A new group of eubacterial light-driven retinal-binding proton pumps with an unusual cytoplasmic proton donor
Author: Harris, Andrew
Department: Department of Physics
Program: Biophysics
Advisor: Brown, Leonid
Abstract: Microbial rhodopsins are a versatile family of photoactive retinal-binding membrane proteins which are widespread geographically and taxonomically. One of the main functions of microbial rhodopsins is outward-directed light-driven proton transport across the plasma membrane, which can provide sources of energy alternative to respiration and chlorophyll photosynthesis. Proton-pumping rhodopsins are found in Archaea (Halobacteria), multiple groups of Bacteria, numerous fungi, and some microscopic algae. This work describes a new group of efficient proteobacterial retinal-binding light-driven proton pumps which lack the carboxylic proton donor on helix C (most often replaced by Gly) but possess a unique His residue on helix B. The typical representative of the group (from Pseudomonas putida) was characterized spectroscopically and through site-directed mutagenesis, which suggested that the unique histidine at position 37 likely forms a proton-donating complex involving water molecules compensating for the loss of the carboxylic proton donor.
URI: http://hdl.handle.net/10214/9666
Date: 2016-05
Rights: Attribution-NonCommercial-NoDerivs 2.5 Canada
Terms of Use: All items in the Atrium are protected by copyright with all rights reserved unless otherwise indicated.


Files in this item

Files Size Format View Description
Harris_Andrew_201605_Msc.pdf 2.795Mb PDF View/Open Master's Thesis

This item appears in the following Collection(s)

Show full item record

Attribution-NonCommercial-NoDerivs 2.5 Canada Except where otherwise noted, this item's license is described as Attribution-NonCommercial-NoDerivs 2.5 Canada