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Characterization of Phytochemicals and Antioxidant Activities of Specialty Tomatoes

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Title: Characterization of Phytochemicals and Antioxidant Activities of Specialty Tomatoes
Author: YUWEI, QI
Department: Department of Food Science
Program: Food Science
Advisor: MINE, YOSHI
Abstract: Twenty-eight specialty tomato cultivars were studied for the phenolic compositions, antioxidant activities in both hydrophilic and hydrophobic fractions of the samples, and the potential effect of simulated in vitro digestion on phenolic composition. Antioxidant activities were evaluated by both chemical-based assays (DPPH, FRAP and ORAC-L/ORAC-H) and cell-based antioxidant assay (CAA). Phenolic compositions were analyzed by HPLC-DAD, which showed that in addition to the phytochemicals commonly known for tomatoes, cultivars such as Lemon Boy Hybrid had a unique composition of phenolics. Cultivar Cuban Yellow Grape showed the highest total phenolic content at 7.12 mg GAE/g DW, and exhibited the highest antioxidant activities from all three chemical-based antioxidant assays. Cultivar Brand Sweet Plum had the highest total carotenoid content (541.13μg/g DW), which did not show corresponding antioxidant activities. Results indicated that phenolic compounds were bioavailable to the cells as demonstrated by the cell-based antioxidant assay. Phenolic compounds, such as rutin and naringenin were reduced by approximately 70% after simulated gastrointestinal digestion, which may be in part due to the interactions between polyphenols and food matrix such as proteins and dietary polysaccharides. This study showed that specialty tomatoes such as Cuban Yellow Grape, with a unique phytochemical composition, possess higher phenolic content and stronger antioxidant activity, therefore can potentially have added health benefits to humans.
Date: 2016-04
Rights: Attribution-NonCommercial-ShareAlike 2.5 Canada
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Attribution-NonCommercial-ShareAlike 2.5 Canada Except where otherwise noted, this item's license is described as Attribution-NonCommercial-ShareAlike 2.5 Canada