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Nanomechanical Measurements of Viscoelastic Properties of Bacterial Cells: Effect of Antimicrobial Peptides and Challenges Associated with Measurements in Buffer

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dc.contributor.advisor Dutcher, John
dc.contributor.author Parg, Richard
dc.date.accessioned 2016-04-11T13:38:54Z
dc.date.available 2016-04-11T13:38:54Z
dc.date.copyright 2016-03
dc.date.created 2016-03-29
dc.date.issued 2016-04-11
dc.identifier.uri http://hdl.handle.net/10214/9585
dc.description.abstract The effectiveness of antimicrobial compounds can be easily screened; however, their mechanism of action is much more difficult to determine. Many compounds act by compromising the mechanical integrity of the bacterial cell, and we have developed an atomic force microscopy (AFM)-based creep-deformation technique to evaluate changes in the time-dependent mechanical properties of bacterial cells upon exposure to antimicrobials. Measurements revealed large changes to the viscoelastic parameters including a distinctive signature for the loss of integrity of the bacterial cell envelope. This technique provides unique insight into the kinetics and action of antimicrobials on bacteria. Initial experiments were performed in Milli-Q water on Pseudomonas aeruginosa PAO1 bacteria, which can withstand large osmotic pressures. Subsequent experiments addressed the challenges of performing AFM creep deformation experiments on live cells in PBS buffer. We describe measurements of, and improvements to, the stability of the experimental set-up in an effort to facilitate these challenging experiments. en_US
dc.language.iso en en_US
dc.rights Attribution-NoDerivs 2.5 Canada *
dc.rights.uri http://creativecommons.org/licenses/by-nd/2.5/ca/ *
dc.subject Atomic Force Microscopy en_US
dc.subject Bacteria en_US
dc.subject Pseudomonas aeruginosa en_US
dc.subject Antibiotics en_US
dc.subject Polymyxin en_US
dc.subject Force Spectroscopy en_US
dc.subject Creep Deformation en_US
dc.subject Viscoelasticity en_US
dc.title Nanomechanical Measurements of Viscoelastic Properties of Bacterial Cells: Effect of Antimicrobial Peptides and Challenges Associated with Measurements in Buffer en_US
dc.type Thesis en_US
dc.degree.programme Biophysics en_US
dc.degree.name Master of Science en_US
dc.degree.department Department of Physics en_US
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Attribution-NoDerivs 2.5 Canada Except where otherwise noted, this item's license is described as Attribution-NoDerivs 2.5 Canada