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Using polymerase chain reaction to characterize recent prey consumption by the slender running crab spider, Tibellus Simon (Araneae: Philodromidae)

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Title: Using polymerase chain reaction to characterize recent prey consumption by the slender running crab spider, Tibellus Simon (Araneae: Philodromidae)
Author: Struger-Kalkman, Brandes
Department: School of Environmental Sciences
Program: Environmental Sciences
Advisor: Schmidt, Jonathan
Abstract: This thesis investigates the use of polymerase chain reaction (PCR) as a tool for characterizing the recent prey consumption of spiders. A methodology using PCR was developed to detect a 543-bp region of DNA from the cytochrome c oxidase subunit I gene of Drosophila suzukii (Matsumura). Feeding experiments involving slender running crab spiders, Tibellus Simon spp., revealed the robust detection of target DNA from the extracts of Tibellus for up to 60 h after one D. suzukii was consumed. Further experiments showed that consuming multiple D. suzukii extended detection to 96 h in Tibellus. A field study demonstrated that the method detected recent predation by 31% of Tibellus collected in the field. However, an evaluation of the specificity of the method determined that modification is necessary for precisely identifying the prey of field spiders. Avenues of future research on spider predation in both the laboratory and field are discussed.
URI: http://hdl.handle.net/10214/9527
Date: 2016-02
Rights: Attribution-NoDerivs 2.5 Canada


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Attribution-NoDerivs 2.5 Canada Except where otherwise noted, this item's license is described as Attribution-NoDerivs 2.5 Canada