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Investigating Hepatic Glutamate Dehydrogenase Activity as a Cause for the Significant Loss of Amino Acid Utilization Efficiency in Growing Rainbow Trout (Oncorhynchus mykiss)

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Title: Investigating Hepatic Glutamate Dehydrogenase Activity as a Cause for the Significant Loss of Amino Acid Utilization Efficiency in Growing Rainbow Trout (Oncorhynchus mykiss)
Author: Chan, Justin Robert
Department: Department of Animal Biosciences
Program: Animal and Poultry Science
Advisor: Bureau, Dominique
Abstract: Rainbow trout (Oncorhynchus mykiss) exhibit an interesting characteristic where their amino acid utilization efficiency progressively and significantly decreases as they increase in size and age; the cause for which is currently unknown. The catabolic enzyme Glutamate Dehydrogenase (GDH) was assayed to compare its activity level in the liver between two age-classes (0+ and 1+). Total activity per liver (U liver-1) was significantly greater (p < 0.0001) in 1+, specific activity (U mg protein-1) was significantly greater (p < 0.009) in 0+, and total activity per gram of liver (U g liver-1) was not significantly different. GDH activity may be responsible for the efficiency loss as total activity per liver increased at a greater rate than body and liver size. A mechanistic, mass-balance model for each age-class was constructed to track amino acid metabolism via nitrogen that attributed catabolism to the total activity on a per liver basis of GDH.
URI: http://hdl.handle.net/10214/9518
Date: 2016-01


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