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An Investigation of the Non-Redundant Functions of PABP4 via Protein-Protein Interactions and PABP-Depletion Studies

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dc.contributor.advisor Bag, Jnanankur
dc.contributor.author Blake, Katherine
dc.date.accessioned 2016-02-05T17:10:24Z
dc.date.available 2016-02-05T17:10:24Z
dc.date.copyright 2016-01
dc.date.created 2016-01-28
dc.date.issued 2016-02-05
dc.identifier.uri http://hdl.handle.net/10214/9517
dc.description.abstract Poly(A) binding proteins are a group of RNA binding proteins with specific affinities towards the poly(A) tract of eukaryotic mRNAs. Although the cellular function of PABP1 in mRNA metabolism is well characterized, the function of other cytoplasmic PABPs is less understood. The aim of this research was to elucidate the cellular function of PABP4. We observed a modest reduction in protein synthesis in PABP4 depleted cells. Additionally, we investigated protein-protein interactions, finding that PABP1 and PABP4 interact with one another. We also found that while PABP4 binds to Paip1, it failed to bind Paip2. Consistent with this observation, PABP1 depletion results in the degradation of Paip2. However, PABP4 depletion had no effect on Paip2 expression. Studies were also conducted to examine PABP1 and PABP4 expression during hypoxia and recovery. Our results did not show any detectable changes in cellular abundance of both PABP1, and PABP4 during hypoxia or recovery. en_US
dc.language.iso en en_US
dc.rights Attribution-NonCommercial-NoDerivs 2.5 Canada *
dc.rights.uri http://creativecommons.org/licenses/by-nc-nd/2.5/ca/ *
dc.subject PABP en_US
dc.subject PABP4 en_US
dc.subject Hypoxia en_US
dc.subject Knockdown en_US
dc.subject Depletion en_US
dc.subject Paip1 en_US
dc.subject Paip2 en_US
dc.title An Investigation of the Non-Redundant Functions of PABP4 via Protein-Protein Interactions and PABP-Depletion Studies en_US
dc.type Thesis en_US
dc.degree.programme Molecular and Cellular Biology en_US
dc.degree.name Master of Science en_US
dc.degree.department Department of Molecular and Cellular Biology en_US
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Attribution-NonCommercial-NoDerivs 2.5 Canada Except where otherwise noted, this item's license is described as Attribution-NonCommercial-NoDerivs 2.5 Canada