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An Investigation of the Non-Redundant Functions of PABP4 via Protein-Protein Interactions and PABP-Depletion Studies

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Title: An Investigation of the Non-Redundant Functions of PABP4 via Protein-Protein Interactions and PABP-Depletion Studies
Author: Blake, Katherine
Department: Department of Molecular and Cellular Biology
Program: Molecular and Cellular Biology
Advisor: Bag, Jnanankur
Abstract: Poly(A) binding proteins are a group of RNA binding proteins with specific affinities towards the poly(A) tract of eukaryotic mRNAs. Although the cellular function of PABP1 in mRNA metabolism is well characterized, the function of other cytoplasmic PABPs is less understood. The aim of this research was to elucidate the cellular function of PABP4. We observed a modest reduction in protein synthesis in PABP4 depleted cells. Additionally, we investigated protein-protein interactions, finding that PABP1 and PABP4 interact with one another. We also found that while PABP4 binds to Paip1, it failed to bind Paip2. Consistent with this observation, PABP1 depletion results in the degradation of Paip2. However, PABP4 depletion had no effect on Paip2 expression. Studies were also conducted to examine PABP1 and PABP4 expression during hypoxia and recovery. Our results did not show any detectable changes in cellular abundance of both PABP1, and PABP4 during hypoxia or recovery.
URI: http://hdl.handle.net/10214/9517
Date: 2016-01
Rights: Attribution-NonCommercial-NoDerivs 2.5 Canada


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Attribution-NonCommercial-NoDerivs 2.5 Canada Except where otherwise noted, this item's license is described as Attribution-NonCommercial-NoDerivs 2.5 Canada