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Investigating the Role of Axin2 in the Regulation of the Wnt Signaling Pathway in Early Zebrafish Development

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dc.contributor.advisor Van Raay, Terry
dc.contributor.author Pasula, Daniel
dc.date.accessioned 2016-01-12T15:16:36Z
dc.date.available 2016-01-12T15:16:36Z
dc.date.copyright 2016-01
dc.date.created 2016-01-06
dc.date.issued 2016-01-12
dc.identifier.uri http://hdl.handle.net/10214/9481
dc.description.abstract The negative feedback inhibitor Axin2 is known to inhibit Wnt signaling by re-enabling the destruction complex’s role in the degradation of cytoplasmic β-catenin. To better understand how Axin2 regulates Wnt signaling, I tested the hypothesis that the role of Axin2 is to mediate temporal control of Wnt signaling and that loss of Axin2 in zebrafish embryos will prolong Wnt signaling, especially in embryos challenged with excess Wnt ligand. The CRISPR/Cas9 genome editing system was used to knock out axin2 in zebrafish embryos. Loss of Axin2 had no obvious effect on the developing zebrafish embryo and there was no evidence of hyperactivated Wnt signaling. Instead, loss of Axin2 appeared to hyper-sensitize the embryos to excess Wnt signaling, resulting in significant death. Contrary to my hypothesis, loss of Axin2 did not affect the duration of Wnt signaling, but instead appeared to control the intensity of the Wnt signal. en_US
dc.language.iso en en_US
dc.rights Attribution-NonCommercial-ShareAlike 2.5 Canada *
dc.rights.uri http://creativecommons.org/licenses/by-nc-sa/2.5/ca/ *
dc.subject Axin2 en_US
dc.subject Wnt en_US
dc.subject Zebrafish en_US
dc.subject CRISPR en_US
dc.title Investigating the Role of Axin2 in the Regulation of the Wnt Signaling Pathway in Early Zebrafish Development en_US
dc.type Thesis en_US
dc.degree.programme Molecular and Cellular Biology en_US
dc.degree.name Master of Science en_US
dc.degree.department Department of Molecular and Cellular Biology en_US
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