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Investigating the Role of Axin2 in the Regulation of the Wnt Signaling Pathway in Early Zebrafish Development

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Title: Investigating the Role of Axin2 in the Regulation of the Wnt Signaling Pathway in Early Zebrafish Development
Author: Pasula, Daniel
Department: Department of Molecular and Cellular Biology
Program: Molecular and Cellular Biology
Advisor: Van Raay, Terry
Abstract: The negative feedback inhibitor Axin2 is known to inhibit Wnt signaling by re-enabling the destruction complex’s role in the degradation of cytoplasmic β-catenin. To better understand how Axin2 regulates Wnt signaling, I tested the hypothesis that the role of Axin2 is to mediate temporal control of Wnt signaling and that loss of Axin2 in zebrafish embryos will prolong Wnt signaling, especially in embryos challenged with excess Wnt ligand. The CRISPR/Cas9 genome editing system was used to knock out axin2 in zebrafish embryos. Loss of Axin2 had no obvious effect on the developing zebrafish embryo and there was no evidence of hyperactivated Wnt signaling. Instead, loss of Axin2 appeared to hyper-sensitize the embryos to excess Wnt signaling, resulting in significant death. Contrary to my hypothesis, loss of Axin2 did not affect the duration of Wnt signaling, but instead appeared to control the intensity of the Wnt signal.
URI: http://hdl.handle.net/10214/9481
Date: 2016-01
Rights: Attribution-NonCommercial-ShareAlike 2.5 Canada
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Attribution-NonCommercial-ShareAlike 2.5 Canada Except where otherwise noted, this item's license is described as Attribution-NonCommercial-ShareAlike 2.5 Canada