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Furan Modified Deoxyuridine Probes For Aptasensor Development And Protein Cross-linking Applications

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Title: Furan Modified Deoxyuridine Probes For Aptasensor Development And Protein Cross-linking Applications
Author: Cservenyi, Thomas
Department: Department of Chemistry
Program: Chemistry
Advisor: Manderville, Richard
Abstract: Here, 2-furanyl deoxyuridine probes were explored for aptasensor applications. The probe was placed into the thrombin binding aptamer (TBA) which is known to fold into a guanine quadruplex (GQ) and bind to a therapeutically relevant protein. In addition, the probe was incorporated into the human telomeric (HTelo) sequence, a repetitive sequence found at the terminal end of chromosomes that has been linked to cancer and aging. Overall, the probe was able to produce diagnostic signals between the conformational heterogeneity of both TBA and HTelo, providing a foundation for future thymine-based probe development. A second focus of this thesis involves protein-DNA conjugation. Forming crosslinks is a desirable technology for applications including disease biomarker discovery or the enhancement of therapeutic effects. The work herein shows how 3-furanyl probes within TBA could be used for future industrial applications because for their ability to form potent, specific crosslinks between the aptamer and thrombin.
URI: http://hdl.handle.net/10214/9478
Date: 2015-12
Rights: Attribution-NonCommercial-NoDerivs 2.5 Canada
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Attribution-NonCommercial-NoDerivs 2.5 Canada Except where otherwise noted, this item's license is described as Attribution-NonCommercial-NoDerivs 2.5 Canada