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Characterization of the interactions between CcmM and Rubisco – key players in organizing the β-carboxysome interior

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dc.contributor.advisor Kimber, Matthew S. Wroblewski, Charles 2016-01-07T21:15:56Z 2016-12-07T06:00:27Z 2015-12 2015-12-07 2016-01-07
dc.description.abstract The key step in carbon fixation is catalyzed by RuBisCO, a multimeric protein comprised of eight large (RbcL) and eight small (RbcS) subunits. Low catalytic turnover rate, low affinity for CO2 and poor discrimination against the counterproductive alternative substrate O2 limits RuBisCO’s catalytic efficiency. Cyanobacteria compensate for RuBisCO’s limitations by utilizing a set of systems known as the Carbon Concentrating Mechanism (CCM) to increase the concentration of CO2 within RuBisCO’s proximity. Carboxysomes are built from thousands of protein subunits arranged in a closed icosahedral shell, permeable to select substrates, with the functional enzymes (RuBisCO and carbonic anhydrase) enclosed within. RuBisCO within the carboxysomes is found in a highly ordered paracrystalline array. CcmM is thought to play a central role in organizing the carboxysome; it is present in two forms, long (CcmM58) and short (CcmM35) which lacks the N-terminal domain found in the long form. The C-terminal domain is built of several repeats homologous to an RbcS-like motif separated by hydrophilic linker regions. The gene encoding the small subunits of Thermosynechococcus elongatus CcmM were subcloned and ligated into the pBTL-4 vector. RuBisCO is encoded by the rbcLXS gene. CcmM small subunits co-purified with RuBisCO, when they were co-expressed in E. coli. The exact nature of the CcmM-RuBisCO complex, the role it plays in both assembly of the carboxysome core and interaction with the protein shell, are all currently unknown. en_US
dc.language.iso en en_US
dc.subject RuBisCO, Carboxysomes, CcmM en_US
dc.title Characterization of the interactions between CcmM and Rubisco – key players in organizing the β-carboxysome interior en_US
dc.type Thesis en_US Molecular and Cellular Biology en_US Master of Science en_US Department of Molecular and Cellular Biology en_US
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