Main content

Epigenetic inheritance in Saccharomyces cerevisiae: the competition between CAF-I and Rrm3p for the DNA sliding clamp PCNA

Show full item record

Title: Epigenetic inheritance in Saccharomyces cerevisiae: the competition between CAF-I and Rrm3p for the DNA sliding clamp PCNA
Author: Wyse, Brandon
Department: Department of Molecular and Cellular Biology
Program: Molecular and Cellular Biology
Advisor: Yankulov, Krassimir
Abstract: Conversions between two epigenetically heritable states (fully active or silent) are regulated by chromatin and integral for development and disease. The mechanisms of epigenetic conversions are not fully elucidated. CAF-I and Rrm3p are key regulators of conversions. I hypothesized that the kinases Cdc7p and Cdc28p phosphorylate CAF-I and facilitate its association with PCNA. Both CAF-I and Rrm3p interact with PCNA through the same domain. Therefore, I hypothesized that CAF-I and Rrm3p compete for PCNA. I used a modified yeast two-hybrid assay to assess the CAF-I/PCNA interaction. This interaction was significantly reduced when Cdc7p phosphorylation of Cac1p is prevented. I used a similar assay to show that CAF-I and Rrm3p compete for PCNA. I also showed differential binding between Cac1p and Rrm3p, which indicated that the PIP box is not the only motif responsible for the interaction. Finally, I proposed and discussed a new model for such conversions.
URI: http://hdl.handle.net/10214/9402
Date: 2015-12
Rights: Attribution-NoDerivs 2.5 Canada


Files in this item

Files Size Format View Description
Wyse_Brandon_20152_Msc.pdf 3.687Mb PDF View/Open Thesis Body

This item appears in the following Collection(s)

Show full item record

Attribution-NoDerivs 2.5 Canada Except where otherwise noted, this item's license is described as Attribution-NoDerivs 2.5 Canada