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Assessing Belowground Plant Diversity in Wetland Soil through DNA Metabarcoding: Impact of DNA Marker Selection and Analysis of Temporal Patterns

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dc.contributor.advisor Hajibabaei, Mehrdad
dc.contributor.author Fahner, Nicole Allison
dc.date.accessioned 2015-12-18T16:46:11Z
dc.date.available 2016-12-10T06:00:15Z
dc.date.copyright 2015-12
dc.date.created 2015-12-10
dc.date.issued 2015-12-18
dc.identifier.uri http://hdl.handle.net/10214/9396
dc.description.abstract This thesis is an investigation of the DNA metabarcoding approach to biodiversity assessment of vascular plant diversity. Specifically, the investigation focused on DNA metabarcoding of environmental DNA extracted from unsorted soil samples. There were two main research goals: to evaluate the suitability of four established DNA marker regions – matK, rbcL, ITS2, and the P6 loop of the trnL intron – for biodiversity assessment of vascular plants and to examine community turnover in total belowground vascular plant diversity. Based on the relative annotation, resolution and recovery ability of the DNA markers, rbcL and ITS2 were recommended for future biodiversity assessments. Annual variability in belowground diversity was consistent in magnitude with previous aboveground observations suggesting that accumulation of plant tissues is not a major restriction for soil-based biodiversity assessments. Finally, an interaction between DNA marker and observed community turnover was identified and positively correlated with length of DNA marker. en_US
dc.description.sponsorship Environment Canada, Parks Canada, NSERC, Genome Canada, Ontario Genomics Institute en_US
dc.language.iso en en_US
dc.rights Attribution 2.5 Canada *
dc.rights.uri http://creativecommons.org/licenses/by/2.5/ca/ *
dc.subject DNA metabarcoding en_US
dc.subject vascular plants en_US
dc.subject DNA barcoding en_US
dc.subject rbcL en_US
dc.subject matK en_US
dc.subject trnL en_US
dc.subject internal transcribed spacer en_US
dc.subject environmental DNA en_US
dc.subject biodiversity assessment en_US
dc.subject community turnover en_US
dc.subject Peace Athabasca Delta en_US
dc.subject belowground en_US
dc.subject Wood Buffalo National Park en_US
dc.subject wetlands en_US
dc.title Assessing Belowground Plant Diversity in Wetland Soil through DNA Metabarcoding: Impact of DNA Marker Selection and Analysis of Temporal Patterns en_US
dc.type Thesis en_US
dc.degree.programme Integrative Biology en_US
dc.degree.name Master of Science en_US
dc.degree.department Department of Integrative Biology en_US
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Attribution 2.5 Canada Except where otherwise noted, this item's license is described as Attribution 2.5 Canada