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Biochemical Characterization of Sinapate Ester Metabolism in Arabidopsis thaliana in Response to Transient Abiotic Stress

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dc.contributor.advisor Bozzo, Gale G. Neil, Kevin J. A. 2015-12-18T14:46:23Z 2016-11-30T06:00:31Z 2015-12 2015-11-30 2015-12-18
dc.description.abstract Flavonol bisglycosides and sinapate esters are metabolites that accumulate in plants with abiotic stress. In Arabidopsis thaliana, flavonol bisglycoside levels increase during nitrogen deficiency and low temperature (NDLT), and are lost during recovery from NDLT. Flavonol 3-O-β-glucoside-7-O-α-rhamnoside catabolism is dependent upon β-glucosidase (i.e., BGLU15) activity. Here, 1-O-sinapoyl-β-D-glucose and 1,2-bis-O-sinapoyl-β-glucoside levels increased in Arabidopsis after 7 d of NDLT, and gradually disappeared within 5 d of recovery. BGLU16 is phylogenetically related to BGLU15, and their transcript levels increased during recovery from NDLT. Following expression in bacteria and purification to homogeneity, biochemical assays revealed that the recombinant BGLU16 utilizes the artificial substrate p-nitrophenyl-β-D-glucoside and quercetin 3-O-β-glucoside; other flavonol glycosides were not hydrolyzed. BGLU16 incubated with an Arabidopsis extract containing phenylpropanoids and flavonols yielded sinapate as the product, implying that BGLU16 hydrolyzes sinapate esters. This information is important for designing biochemical studies aimed at elucidating the relevance of sinapate ester catabolism in plants. en_US
dc.language.iso en en_US
dc.rights Attribution-NonCommercial-ShareAlike 2.5 Canada *
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dc.subject biochemistry en_US
dc.subject secondary metabolism en_US
dc.subject Arabidopsis en_US
dc.subject phenylpropanoid en_US
dc.subject sinapate esters en_US
dc.subject stress en_US
dc.subject stress metabolism en_US
dc.subject plant molecular biology en_US
dc.subject protein expression en_US
dc.subject protein purification en_US
dc.title Biochemical Characterization of Sinapate Ester Metabolism in Arabidopsis thaliana in Response to Transient Abiotic Stress en_US
dc.type Thesis en_US Plant Agriculture en_US Master of Science en_US Department of Plant Agriculture en_US
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