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Biochemical Characterization of Sinapate Ester Metabolism in Arabidopsis thaliana in Response to Transient Abiotic Stress

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Title: Biochemical Characterization of Sinapate Ester Metabolism in Arabidopsis thaliana in Response to Transient Abiotic Stress
Author: Neil, Kevin J. A.
Department: Department of Plant Agriculture
Program: Plant Agriculture
Advisor: Bozzo, Gale G.
Abstract: Flavonol bisglycosides and sinapate esters are metabolites that accumulate in plants with abiotic stress. In Arabidopsis thaliana, flavonol bisglycoside levels increase during nitrogen deficiency and low temperature (NDLT), and are lost during recovery from NDLT. Flavonol 3-O-β-glucoside-7-O-α-rhamnoside catabolism is dependent upon β-glucosidase (i.e., BGLU15) activity. Here, 1-O-sinapoyl-β-D-glucose and 1,2-bis-O-sinapoyl-β-glucoside levels increased in Arabidopsis after 7 d of NDLT, and gradually disappeared within 5 d of recovery. BGLU16 is phylogenetically related to BGLU15, and their transcript levels increased during recovery from NDLT. Following expression in bacteria and purification to homogeneity, biochemical assays revealed that the recombinant BGLU16 utilizes the artificial substrate p-nitrophenyl-β-D-glucoside and quercetin 3-O-β-glucoside; other flavonol glycosides were not hydrolyzed. BGLU16 incubated with an Arabidopsis extract containing phenylpropanoids and flavonols yielded sinapate as the product, implying that BGLU16 hydrolyzes sinapate esters. This information is important for designing biochemical studies aimed at elucidating the relevance of sinapate ester catabolism in plants.
URI: http://hdl.handle.net/10214/9395
Date: 2015-12
Rights: Attribution-NonCommercial-ShareAlike 2.5 Canada
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Attribution-NonCommercial-ShareAlike 2.5 Canada Except where otherwise noted, this item's license is described as Attribution-NonCommercial-ShareAlike 2.5 Canada