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INVESTIGATION OF GENOME VARIATIONS AS POTENTIAL MARKERS FOR BOAR FERTILITY

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dc.contributor.advisor King, W Allan
dc.contributor.author Quach, Anh
dc.date.accessioned 2015-09-22T20:22:36Z
dc.date.available 2015-09-22T20:22:36Z
dc.date.copyright 2015-09
dc.date.created 2015-09-18
dc.date.issued 2015-09-22
dc.identifier.uri http://hdl.handle.net/10214/9269
dc.description.abstract Boars can greatly affect the reproductive success of a herd, especially with the current widespread use of artificial insemination (AI). Low fertility boars in AI centers can cause enormous economic losses. Thus, identifying a method to detect low fertility boars at an early stage of their life will increase profitability for the swine industry. The purposes of this thesis were to investigate genomic variations including chromosome abnormalities, copy number variation (CNV) and a very special case of CNV on the Y chromosome, testis specific protein Y-encoded (TSPY) gene in pig populations to develop and assess significant tools for detecting low fertility boars. These assessments included karyotyping all young AI boars and evaluating whether CNV of some genes can be used as genetic markers of male fertility in pig. At the chromosome level, this study identified 12 different chromosome rearrangements including 9 reciprocal translocations, two inversions and one Robertsonian translocation. The large adverse effect on litter size of carriers of chromosome abnormalities together with the high prevalence of chromosome abnormalities (1.64%) and high percentage of de novo cases (2/5 cases) in the Canadian pig populations highlights the significant value of having an imperative screening program for chromosome abnormalities in Canadian swine industry. At the DNA level, two groups of animals with direct boar effects (DBE) values in the upper (high fertility) and lower (low fertility) end of the distribution were used for CNV analysis and the identification of putative markers of fertility. This study identified 35 CNV regions (CNVRs) covering 36.5 Mb or ~1.3% of the porcine genome. The identified 35 CNVRs encompassed 50 genes. A functional analysis of several databases revealed that the genes found in CNVRs from the low fertility group have been significantly enriched in members of the innate immune system, Toll-like receptor and RIG-I-like receptor signaling and fatty acid oxidation pathways. Unlike humans and cattle, boars have only 3 copies of TSPY gene that do not vary among individuals thus copy number of TSPY gene represents a poor marker for fertility in pig. en_US
dc.description.sponsorship Vietnamese Overseas Scholarship Program, Canadian Centre for Swine Improvement, Agricultural Adaptation Council, NSERC, CRC en_US
dc.language.iso en en_US
dc.subject Chromosome abnormality en_US
dc.subject Copy number variation en_US
dc.subject TSPY en_US
dc.subject boar fertility en_US
dc.title INVESTIGATION OF GENOME VARIATIONS AS POTENTIAL MARKERS FOR BOAR FERTILITY en_US
dc.type Thesis en_US
dc.degree.programme Biomedical Sciences en_US
dc.degree.name Doctor of Philosophy en_US
dc.degree.department Department of Biomedical Sciences en_US


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