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Quantifying Spatiotemporal Patterns in the Expansion of Twitching Bacterial Colonies

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dc.contributor.advisor Dutcher, John Shelton, Erin 2015-09-08T18:58:12Z 2015-09-08T18:58:12Z 2015-08 2015-08-31 2015-09-08
dc.description.abstract Type IV pili (T4P) are protein filaments used by certain Gram-negative bacteria for a mode of surface motility called twitching. We have developed experimental and data analysis techniques to quantify the expansion of twitching P. aeruginosa colonies at an agar-glass interface as a function of agar concentration C. The advancing front consists of finger-like protrusions of bacteria, with cells in the expanding colony arranged in a lattice-like pattern. We find that the average finger width increases linearly with C, whereas the average finger speed is independent of C. This latter result, coupled with the observed transition from monolayer to multilayer coverage within fingers, suggests a critical number of T4P are required to break through the agar-glass interface. In addition, we have calculated line profiles across fingers, quantifying their average speed and bacterial orientation, and performed high spatial and temporal resolution studies of the fingers. en_US
dc.language.iso en en_US
dc.publisher University of Guelph en_US
dc.subject collective motion en_US
dc.subject bacterial twitching motility en_US
dc.subject type iv pili en_US
dc.subject agar stiffness en_US
dc.title Quantifying Spatiotemporal Patterns in the Expansion of Twitching Bacterial Colonies en_US
dc.type Thesis en_US Biophysics en_US Master of Science en_US Department of Physics en_US
dc.rights.license All items in the Atrium are protected by copyright with all rights reserved unless otherwise indicated. University of Guelph en_US

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