Main content

Investigations on Mitochondrial Pleomorphy and Interactions with the Endoplasmic Reticulum and Peroxisomes

Show full item record

Title: Investigations on Mitochondrial Pleomorphy and Interactions with the Endoplasmic Reticulum and Peroxisomes
Author: Jaipargas, Erica-Ashley
Department: Department of Molecular and Cellular Biology
Program: Molecular and Cellular Biology
Advisor: Mathur, Jaideep
Abstract: Mitochondria are pleomorphic organelles capable of constant fusion and fission. Live fluorescent microscopy was employed to investigate mitochondrial pleomorphy during fluctuations in light, sugar and O2. Light and sugar are shown to induce fission whereas a green-to-red photo-convertible mEos fluorescent protein targeted to mitochondria (mitoEos) reveals that hypoxia induces fusion, leading to giant mitochondria. The endoplasmic reticulum (ER) is shown to be a mediator of mitochondrial fission and acts as a mould for morphological transitions displayed by mitochondria. Simultaneous live-imaging also reveals sustained mitochondria-peroxisome interactions, which are apparent in anisotropy1, a cell wall mutant shown here to be light sensitive. Triple transgenic lines were created to differentially label mitochondria, peroxisomes and the ER and show that the ER cages the other organelles including chloroplasts during these sustained interactions. Together, simultaneous live-imaging of mitochondria, peroxisomes and the ER using double and triple Arabidopsis thaliana transgenics further illuminate organelle pleomorphy and interactivity.
URI: http://hdl.handle.net/10214/9093
Date: 2015-08


Files in this item

Files Size Format View Description
Jaipargas_Erica-Ashley_201508_Msc.pdf 4.317Mb PDF View/Open Thesis
Movie 3.1 Mitoc ... bmergence (elm1-1 RER).mov 9.965Mb QuickTime video View/Open Supplementary Movie 3.1
Movie 3.2 Giant mitochondrial fusion.mov 68.39Mb QuickTime video View/Open Supplementary Movie 3.2
Movie 3.3 ER moulding giant mitochondria.avi 3.294Mb Unknown View/Open Supplementary Movie 3.3
Movie 3.4 ER-mediated mitochondrial fission.mov 51.23Mb QuickTime video View/Open Supplementary Movie 3.4
Movie 3.5 ER ca ... ondria and peroxisomes.mov 2.517Mb QuickTime video View/Open Supplementary Movie 3.5
Movie 3.6 Susta ... eroxisome interactions.mov 2.621Mb QuickTime video View/Open Supplementary Movie 3.6
Movie 3.7 Fragm ... ia clustering peroxule.mov 8.621Mb QuickTime video View/Open Supplementary Movie 3.7
Movie 3.8 Abundant peroxules in any1.mov 2.052Mb QuickTime video View/Open Supplementary Movie 3.8

This item appears in the following Collection(s)

Show full item record