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Investigations on Mitochondrial Pleomorphy and Interactions with the Endoplasmic Reticulum and Peroxisomes

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Title: Investigations on Mitochondrial Pleomorphy and Interactions with the Endoplasmic Reticulum and Peroxisomes
Author: Jaipargas, Erica-Ashley
Department: Department of Molecular and Cellular Biology
Program: Molecular and Cellular Biology
Advisor: Mathur, Jaideep
Abstract: Mitochondria are pleomorphic organelles capable of constant fusion and fission. Live fluorescent microscopy was employed to investigate mitochondrial pleomorphy during fluctuations in light, sugar and O2. Light and sugar are shown to induce fission whereas a green-to-red photo-convertible mEos fluorescent protein targeted to mitochondria (mitoEos) reveals that hypoxia induces fusion, leading to giant mitochondria. The endoplasmic reticulum (ER) is shown to be a mediator of mitochondrial fission and acts as a mould for morphological transitions displayed by mitochondria. Simultaneous live-imaging also reveals sustained mitochondria-peroxisome interactions, which are apparent in anisotropy1, a cell wall mutant shown here to be light sensitive. Triple transgenic lines were created to differentially label mitochondria, peroxisomes and the ER and show that the ER cages the other organelles including chloroplasts during these sustained interactions. Together, simultaneous live-imaging of mitochondria, peroxisomes and the ER using double and triple Arabidopsis thaliana transgenics further illuminate organelle pleomorphy and interactivity.
URI: http://hdl.handle.net/10214/9093
Date: 2015-08
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Jaipargas_Erica-Ashley_201508_Msc.pdf 4.317Mb PDF View/Open Thesis
Movie_3.1_Mitoc ... bmergence_(elm1-1_RER).mov 9.965Mb QuickTime video View/Open Supplementary Movie 3.1
Movie_3.2_Giant_mitochondrial_fusion.mov 68.39Mb QuickTime video View/Open Supplementary Movie 3.2
Movie_3.3_ER_moulding_giant_mitochondria.avi 3.294Mb Unknown View/Open Supplementary Movie 3.3
Movie_3.4_ER-mediated_mitochondrial_fission.mov 51.23Mb QuickTime video View/Open Supplementary Movie 3.4
Movie_3.5_ER_ca ... ondria_and_peroxisomes.mov 2.517Mb QuickTime video View/Open Supplementary Movie 3.5
Movie_3.6_Susta ... eroxisome_interactions.mov 2.621Mb QuickTime video View/Open Supplementary Movie 3.6
Movie_3.7_Fragm ... ia_clustering_peroxule.mov 8.621Mb QuickTime video View/Open Supplementary Movie 3.7
Movie_3.8_Abundant_peroxules_in_any1.mov 2.052Mb QuickTime video View/Open Supplementary Movie 3.8

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