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Aptasensor Development For Biomarker Detection Using Fluorescent Guanine Quadruplexes

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dc.contributor.advisor Manderville, Richard A. Blanchard, Darian 2015-07-30T18:38:17Z 2016-07-22T05:00:45Z 2015-07 2015-07-22 2015-07-30
dc.description.abstract Aptamers are nucleic acids that are found to bind molecular targets with a high degree of specificity and affinity. Guanine (G) rich aptamers are known to fold into G quadruplex structures (GQ). The synthesis of fluorescently modified guanine probes, 8-(4ʹʹ-acetylstyryl)-2ʹ-dG (AcVdG) and 8-((2-benzo[b]thiophen-2-yl)vinyl)-2ʹ-dG VBTdG, are presented in this thesis. The synthesized 8-vinyl-aryl-probes were incorporated into the TBA using solid phase oligonucleotide synthesis. Their impact on DNA structures were explored as was the ability of the probes to be used for the fluorescent detection of Pb2+, Sr2+, and Ba2+. Furthermore, to utilize the benefits of 8-aryl-dG and 8-vinyl-aryl-dG probes, both were incorporated into the TBA. The two probes act as Fluorescent Resonance Energy Transfer (FRET) pairs; one was used as the “switch” in the system, sensitively detecting GQ folding, whiles the other acts as “the signal” or “light” producing a detectable signal in the visible region. en_US
dc.description.sponsorship Grain Farmers of Ontario, Growing Forward 2, NSERC. Ontario Government en_US
dc.language.iso en en_US
dc.subject Fluorescent Probes en_US
dc.subject Synthetic DNA en_US
dc.subject Modified nucleobases en_US
dc.subject Guanine Quadruplexes en_US
dc.subject Toxin Detection en_US
dc.subject Aptamers en_US
dc.title Aptasensor Development For Biomarker Detection Using Fluorescent Guanine Quadruplexes en_US
dc.type Thesis en_US Chemistry en_US Bachelor of Science en_US Department of Chemistry en_US
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