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Expression of biofilm associated genes in Staphylococcus pseudintermedius

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dc.contributor.advisor Singh, Ameet
dc.contributor.author Crawford, Evan Cameron
dc.date.accessioned 2015-06-25T15:41:30Z
dc.date.available 2015-06-25T15:41:30Z
dc.date.copyright 2015-06
dc.date.created 2015-06-15
dc.date.issued 2015-06-25
dc.identifier.uri http://hdl.handle.net/10214/8935
dc.description.abstract Staphylococcus pseudintermedius is a common commensal organism of canines that has emerged and disseminated internationally as an important pathogen. The biofilm forming ability of this bacterium is increasingly appreciated as a virulence factor, especially in surgical site and implant associated infections, where the formation of biofilms results in persistent, recurrent infections that are often recalcitrant to treatment. This thesis is an investigation into the genetic basis of biofilm formation in Staphylococcus pseudintermedius. The initial step was the development and validation of a quantitative PCR test including the identification of suitable reference genes in this species. Subsequently, that test was used to examine the expression of multiple biofilm associated genes responsible for numerous aspect of biofilm development and maturation. Significant variations in expression levels between surfaces were identified, providing potential insights into several clinical situations. This work will form the foundation for future studies examining specific details of biofilm formation and development, as well as studies directly examining clinical formed biofilms. en_US
dc.description.sponsorship American Kennel Club Canine Health Foundation ACORN grant number 02083-A, University of Guelph Ontario Veterinary College Pet Trust en_US
dc.language.iso en en_US
dc.rights Attribution-NonCommercial-NoDerivs 2.5 Canada *
dc.rights.uri http://creativecommons.org/licenses/by-nc-nd/2.5/ca/ *
dc.subject biofilm en_US
dc.subject MRSP en_US
dc.subject gene expression en_US
dc.subject qPCR en_US
dc.subject surgical site infection en_US
dc.title Expression of biofilm associated genes in Staphylococcus pseudintermedius en_US
dc.type Thesis en_US
dc.degree.programme Clinical Studies en_US
dc.degree.name Doctor of Veterinary Science en_US
dc.degree.department Department of Clinical Studies en_US


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Attribution-NonCommercial-NoDerivs 2.5 Canada Except where otherwise noted, this item's license is described as Attribution-NonCommercial-NoDerivs 2.5 Canada