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Determining the Structure and Orientation of the Alamethicin Ion Channel in Lipid Membranes using ATR and Electrochemical PMIRRAS

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dc.contributor.advisor Lipkowski, Jacek
dc.contributor.author Shodiev, Muzaffar
dc.date.accessioned 2015-05-05T15:15:28Z
dc.date.available 2015-05-05T15:15:28Z
dc.date.copyright 2015-05
dc.date.created 2015-05-01
dc.date.issued 2015-05-05
dc.identifier.uri http://hdl.handle.net/10214/8800
dc.description.abstract Alamethicin is a short antibiotic peptide produced by the fungus Trichoderma viride. Ion channels formed by Alamethicin are voltage-gated due to their large molecular dipoles. The goals of this project were to monitor the incorporation of Alamethicin into 1,2-diphytanoyl-sn-glycero-3-phosphocholine (DPhPC) model biomimetic membranes and characterize the opening and closing of these incorporated Alamethicin channels. Attenuated Total Reflection Fourier Transform Infrared spectroscopy (ATR-FTIR) was employed to elucidate the mechanism of Alamethicin incorporation by first spreading vesicles onto the ATR prism. The IR signature of Alamethicin was determined and different peptide ratios were tested. In order to determine the opening and closing of the Alamethicin pore, a bilayer containing Alamethicin was deposited onto the surface of a thioglucose modified Au(111) electrode. Polarization Modulation Infrared Reflection Absorption Spectroscopy (PMIRRAS) was used to examine changes in the structure and orientation of Alamethicin as a function of the applied potential. en_US
dc.language.iso en en_US
dc.rights Attribution-NonCommercial-NoDerivs 2.5 Canada *
dc.rights.uri http://creativecommons.org/licenses/by-nc-nd/2.5/ca/ *
dc.subject Alamethicin en_US
dc.subject DPhPC en_US
dc.subject PMIRRAS en_US
dc.subject ATR-FTIR en_US
dc.subject Bilayer lipid membrane en_US
dc.subject Peptide insertion en_US
dc.subject IR Spectroscopy en_US
dc.subject Orientation and conformation en_US
dc.subject Applied electric field en_US
dc.subject Electrochemical cell en_US
dc.title Determining the Structure and Orientation of the Alamethicin Ion Channel in Lipid Membranes using ATR and Electrochemical PMIRRAS en_US
dc.type Thesis en_US
dc.degree.programme Chemistry en_US
dc.degree.name Bachelor of Science en_US
dc.degree.department Department of Chemistry en_US
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Attribution-NonCommercial-NoDerivs 2.5 Canada Except where otherwise noted, this item's license is described as Attribution-NonCommercial-NoDerivs 2.5 Canada