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Development and Application of Differential Ion Mobility and Mass Spectrometry for the Analysis of Monosaccharides

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dc.contributor.advisor Gabryelski, Wojciech
dc.contributor.advisor Monteiro, Mario
dc.contributor.author Man, Dorothy
dc.date.accessioned 2015-05-05T13:52:00Z
dc.date.available 2015-05-05T13:52:00Z
dc.date.copyright 2015-04
dc.date.created 2015-04-30
dc.date.issued 2015-05-05
dc.identifier.uri http://hdl.handle.net/10214/8797
dc.description.abstract In carbohydrate analysis, challenges arise in the determination of monosaccharide composition due to the isobaric nature of the molecules. Current conventional techniques such as GC and LC strive to separate isomers of monosaccharides but have been shown to be inefficient and difficult, especially when analyzing underivatized forms. In this thesis, a methodology using differential ion mobility (FAIMS) and mass spectrometry was developed to study the fundamental capabilities of this method for the analysis of underivatized monosaccharides in positive and negative ionization modes. Under the optimal conditions established, the investigation of 6 monosaccharides led to the discovery of a wide range of ions originating from single monosaccharide samples, which were then assessed for isomer separation. This thesis provides proof of principle demonstrating differential ion mobility as a technique capable of rapid separation of isomeric monosaccharides otherwise unachievable by conventional methods. en_US
dc.language.iso en en_US
dc.rights Attribution-NonCommercial-NoDerivs 2.5 Canada *
dc.rights.uri http://creativecommons.org/licenses/by-nc-nd/2.5/ca/ *
dc.subject Mass Spectrometry en_US
dc.subject Differential Ion Mobility en_US
dc.subject FAIMS en_US
dc.subject Monosaccharide en_US
dc.subject Isomer separation en_US
dc.title Development and Application of Differential Ion Mobility and Mass Spectrometry for the Analysis of Monosaccharides en_US
dc.type Thesis en_US
dc.degree.programme Chemistry en_US
dc.degree.name Master of Science en_US
dc.degree.department Department of Chemistry en_US
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Attribution-NonCommercial-NoDerivs 2.5 Canada Except where otherwise noted, this item's license is described as Attribution-NonCommercial-NoDerivs 2.5 Canada