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Development and Application of Differential Ion Mobility and Mass Spectrometry for the Analysis of Monosaccharides

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Title: Development and Application of Differential Ion Mobility and Mass Spectrometry for the Analysis of Monosaccharides
Author: Man, Dorothy
Department: Department of Chemistry
Program: Chemistry
Advisor: Gabryelski, WojciechMonteiro, Mario
Abstract: In carbohydrate analysis, challenges arise in the determination of monosaccharide composition due to the isobaric nature of the molecules. Current conventional techniques such as GC and LC strive to separate isomers of monosaccharides but have been shown to be inefficient and difficult, especially when analyzing underivatized forms. In this thesis, a methodology using differential ion mobility (FAIMS) and mass spectrometry was developed to study the fundamental capabilities of this method for the analysis of underivatized monosaccharides in positive and negative ionization modes. Under the optimal conditions established, the investigation of 6 monosaccharides led to the discovery of a wide range of ions originating from single monosaccharide samples, which were then assessed for isomer separation. This thesis provides proof of principle demonstrating differential ion mobility as a technique capable of rapid separation of isomeric monosaccharides otherwise unachievable by conventional methods.
URI: http://hdl.handle.net/10214/8797
Date: 2015-04
Rights: Attribution-NonCommercial-NoDerivs 2.5 Canada


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Attribution-NonCommercial-NoDerivs 2.5 Canada Except where otherwise noted, this item's license is described as Attribution-NonCommercial-NoDerivs 2.5 Canada