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Investigating the Function of the Arabidopsis thaliana INDETERMINATE DOMAIN2 gene

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dc.contributor.advisor Colasanti, Joseph Meeks, Tylar 2015-05-01T18:04:01Z 2015-05-01T18:04:01Z 2015-04 2015-04-29 2015-05-01
dc.description.abstract The INDETERMINATE DOMAIN (IDD) genes in Arabidopsis thaliana encode a transcription factor family found in all higher plants. IDD2, one of 16 IDD family genes in Arabidopsis, is highly expressed in hypocotyls, shoot apices and petioles. Loss-of-function idd2 mutants show no obvious differences in growth and development under a variety of conditions, suggesting that IDD2 may function redundantly. Constitutive overexpression of IDD2 causes altered seed coat morphology and defects in early growth. 35S::IDD2 overexpression seedlings show a constitutive response to the phytohormone auxin. Elucidation of IDD2 expression in IDD2::GFP:GUS reporter plants revealed a highly specific pattern, including expression in leaf and cotyledon vasculature, petioles, hypocotyl, shoot apex, root tip and emerging lateral roots, which all correspond to areas with high levels of auxin. Taken together, analysis of loss-of-function mutants, overexpression lines and the IDD2 expression pattern suggests that IDD2 has a redundant role in auxin sensing and response. en_US
dc.description.sponsorship OMAFRA-UofG HQP Program, NSERC en_US
dc.language.iso en en_US
dc.subject Auxin sensing en_US
dc.subject Transcription factor family en_US
dc.subject Arabidopsis en_US
dc.subject seed oil mobilization en_US
dc.subject transcription factor complex en_US
dc.subject IDD en_US
dc.title Investigating the Function of the Arabidopsis thaliana INDETERMINATE DOMAIN2 gene en_US
dc.type Thesis en_US Molecular and Cellular Biology en_US Master of Science en_US Department of Molecular and Cellular Biology en_US
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