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Development of A Liquid Micropropagation System for Asparagus officinalis L.

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Title: Development of A Liquid Micropropagation System for Asparagus officinalis L.
Author: Chen, Xinyu
Department: Department of Plant Agriculture
Program: Plant Agriculture
Advisor: Wolyn, David
Abstract: Asparagus officinalis L. is a dioecious plant that is routinely micropropagated in vitro on solid medium, but efficiency is not optimum, especially for recalcitrant genotypes. Liquid culture systems have been used for other plants, showing improved explant morphology and high multiplication rates. Thus, growth and overall propagation efficiency of asparagus may be improved using liquid culture. The objectives of this research were to test the effects of basal media (DKW, MS), NAA and kinetin concentrations, and concentrations of carbohydrates (glucose, fructose, sucrose, maltose) and growth retardants (ancymidol, paclobutrazol) on shoot growth in liquid media. Nodal segments in DKW medium with 0.5 µM kinetin and 0.125 µM NAA, 87.6 mM glucose and 0.5 µM ancymidol produced a high percentage of shoot induction, and thick shoots with a large number of nodes. The optimal liquid rooting medium was modified MS medium with 10 µM IBA, resulting in 40% of plantlets rooted.
URI: http://hdl.handle.net/10214/8729
Date: 2015-02
Rights: Attribution-NonCommercial-NoDerivs 2.5 Canada
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Attribution-NonCommercial-NoDerivs 2.5 Canada Except where otherwise noted, this item's license is described as Attribution-NonCommercial-NoDerivs 2.5 Canada