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Characterization of a Putative Yield-related Gene in Common Bean (Phaseolus vulgaris L.)

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dc.contributor.advisor Peter, Pauls
dc.contributor.advisor Steven, Rothstein
dc.contributor.author QI, YANZHOU
dc.date.accessioned 2015-02-06T19:31:50Z
dc.date.available 2015-02-06T19:31:50Z
dc.date.copyright 2015-01
dc.date.created 2015-01-27
dc.date.issued 2015-02-06
dc.identifier.uri http://hdl.handle.net/10214/8727
dc.description.abstract Yield is a complex trait for breeders to improve, but the identification of yield-related genes and markers for yield genes may help breeders to select for high-yielding bean varieties. BnMicEmUp (a homolog of AT1G74730 from Arabidopsis) is a gene that is upregulated during microspore embryogenesis in Brassica napus and has been characterized as a transcription factor (bZIP) whose expression is negatively related to seed yield in Arabidopsis. The current study characterized a BnMicEmUp/AT1G74730 homolog (named Phvul.009G190100) in common bean (Phaseolus vulgaris. L), an important food crop throughout the world. The gene was isolated and sequenced from P. vulgaris variety “Lightning” by PCR and RT-PCR using primers designed from the sequence in the bean genome (Phytozome v10) annotated as Phvul.009G190100. The high similarity of gene sequence and protein structure between Phvul.009G190100 and BnMicEmUps suggests that Phvul.009G190100 encodes a transcription factor (cbZIP) that is localized in chloroplasts. Expression levels of Phvul.009G190100 in leaves of a number of bean varieties tended to be negatively related with the seed yield, but the correlation was not statistically significant. Homologs for Phvul.009G190100 were also identified in soybean, which is closely related to common bean. The two soybean homologues (Glyma04g34330 and Glyma06g20240) were found to be in close vicinity of molecular markers that are linked to a large number of yield-related QTLs. It appears that Phvul.009G190100 from P. vulgaris, AT1G74730 from Arabidopsis, BnMicEmUp from B. napus, and Glyma04g34330 and Glyma06g20240 from Glycine max all belong to a gene family that may affect seed yield in plants. en_US
dc.language.iso en en_US
dc.rights Attribution-NonCommercial-NoDerivs 2.5 Canada *
dc.rights.uri http://creativecommons.org/licenses/by-nc-nd/2.5/ca/ *
dc.subject common bean en_US
dc.subject gene expression en_US
dc.subject transcription factor en_US
dc.subject yield-related gene en_US
dc.title Characterization of a Putative Yield-related Gene in Common Bean (Phaseolus vulgaris L.) en_US
dc.type Thesis en_US
dc.degree.programme Plant Agriculture en_US
dc.degree.name Master of Science en_US
dc.degree.department Department of Plant Agriculture en_US
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Attribution-NonCommercial-NoDerivs 2.5 Canada Except where otherwise noted, this item's license is described as Attribution-NonCommercial-NoDerivs 2.5 Canada