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In Vitro Assessment of Saccharomyces cerevisiae Derivatives using Bovine Epithelail Cells, Macrophages, and Mycobacterium avium spp. paratuberculosis

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Title: In Vitro Assessment of Saccharomyces cerevisiae Derivatives using Bovine Epithelail Cells, Macrophages, and Mycobacterium avium spp. paratuberculosis
Author: Li, Ziwei
Department: Department of Animal and Poultry Science
Program: Animal and Poultry Science
Advisor: Karrow, Niel
Abstract: Since yeast Saccharomyces cerevisiae and its cell wall components (CWCs) are being used for the prevention and treatment of enteric diseases in different species, they may also be useful for preventing Johne’s disease (JD), a chronic inflammatory bowel disease of ruminants caused by Mycobacterium avium spp. paratuberculosis (MAP). Therefore, the purpose of this thesis was to assess potential anti-MAP adhesive and immnomodulatory properties of certain S. cerevisiae derivatives. The adherence of mCherry-labeled MAP to bovine mammary epithelial cells (MAC-T) and bovine primary epithelial cells (BECs) co-cultured with yeast CWCs from two different yeast strains A and B, and two forms of dead yeast from strain A were investigated. Several macrophage function-related parameters such as phagocytic activity, reactive oxygen species (ROS) production, and immune-related gene expression were also measured using a bovine macrophage cell line (BOMACs) in the presence of these yeast derivatives. Results demonstrated that these yeast derivatives reduced MAP adhesion to both MAC-T cells and BECs as well as MAP uptake by BOMACs in a concentration-dependent manner after 6-hr exposure. ROS production by BOMACs was increased in response to 6-hr exposure to yeast derivatives. The expression of immune-related genes was also altered after 6 and 24-hr exposure to these yeast derivatives. Overall, this thesis provided insight into various aspects of yeast derivative immunomodulatory bioactivity, and demonstrated in vitro evidence that they may help to prevent MAP infection.
Description: The following thesis has looked at the potential anti-adhesive and immunomodulatory properties of Saccharomyces cerevisiae derivatives by using in vitro bovine epithelial cells, bovine macrophages and Mycobacterium avium spp. paratuberculosis.
URI: http://hdl.handle.net/10214/8696
Date: 2015-01
Rights: Attribution-NonCommercial-NoDerivs 2.5 Canada


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Attribution-NonCommercial-NoDerivs 2.5 Canada Except where otherwise noted, this item's license is described as Attribution-NonCommercial-NoDerivs 2.5 Canada