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Characterizing the Role of Nkd1 in Colorectal Cancer

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Title: Characterizing the Role of Nkd1 in Colorectal Cancer
Author: Kondra, Roman
Department: Department of Molecular and Cellular Biology
Program: Molecular and Cellular Biology
Advisor: Van Raay, Terry
Abstract: The Wnt signaling pathway is a highly conserved pathway critical for development and homeostasis of stem cells. Aberrant Wnt signaling causes developmental defects and disease. Over 90% of colorectal cancers (CRC) contain mutations in Wnt signaling which constitutively activate the Wnt pathway independent of a Wnt ligand. Nkd1 is a negative feedback regulator of Wnt signaling and functions by binding and preventing nuclear accumulation of the central signaling component, β-catenin. Nkd1 is upregulated in a large number of CRC but its function in CRC's is unknown. As NKD1 function is dependent on membrane localization it is hypothesized that Nkd1 is activated by the binding of a Wnt ligand to its receptors. To explore NKD1's function and activation, cancerous and mammalian cell lines were used to examine NKD1's response to Wnt. Results show that, under Wnt stimulation the distribution of NKD1 changes and interaction between NKD1 and β-catenin increases.
URI: http://hdl.handle.net/10214/8682
Date: 2014-11
Rights: Attribution-ShareAlike 2.5 Canada
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Attribution-ShareAlike 2.5 Canada Except where otherwise noted, this item's license is described as Attribution-ShareAlike 2.5 Canada