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Surface Analytical Studies of Gramicidin A in Biomimetic Model Membrane Systems

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Title: Surface Analytical Studies of Gramicidin A in Biomimetic Model Membrane Systems
Author: Goodall, Barbara Lynn
Department: Department of Chemistry
Program: Chemistry
Advisor: Lipkowski, Jacek
Abstract: This thesis is an investigation of the properties of gramicidin A in model membrane systems. This study investigates a solid supported bilayer lipid membrane comprised of 1,2-dimyristoyl-sn-glycero-3-phosphocholine (DMPC) and gramicidin A deposited on a thioglucose modified Au(111) electrode. A “floating” bilayer lipid membrane was also investigated that was composed of DMPC, cholesterol, glycolipid monosialoganglioside (GM1), and gramicidin A deposited on a thioglucose modified Au(111) electrode. The GM1 was used as a spacer molecule to create a water reservoir between the modified electrode and lipid bilayer. Polarization modulation infrared reflection adsorption spectroscopy (PM-IRRAS) was used to investigate the solid supported and the floating bilayer lipid membrane. The PM-IRRAS studies were completed to probe the electric field driven changes in the orientation and conformation of gramicidin A in the lipid bilayer. Atomic force microscopy was used to investigate the topography and thickness of the floating bilayer lipid membrane system.
URI: http://hdl.handle.net/10214/8635
Date: 2014-12
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