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Investigation into the Molecular and Biochemical Mechanisms of Resistance in Two Biotypes of Glyphosate Resistant Giant Ragweed

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Title: Investigation into the Molecular and Biochemical Mechanisms of Resistance in Two Biotypes of Glyphosate Resistant Giant Ragweed
Author: Jeffery, Taylor
Department: Department of Plant Agriculture
Program: Plant Agriculture
Advisor: Tardif, FrançoisSikkema, Peter
Abstract: Glyphosate resistant giant ragweed poses a significant threat to farmers in southern Ontario as interference from this species can result in massive yield loses. The objective of this study is to investigate two distinct mechanisms of glyphosate resistance present in giant ragweed biotypes. Target site sequencing along with inter-population crosses were performed to obtain insight into the pattern of inheritance. The role of H2O2 was examined using 3,3’-Diaminobenzidine staining and glyoxylate content was measured as a means of investigating glycolate oxidase activity. Sequencing data revealed no target site mutations while F1 survival data showed a decreased resistance level. DAB staining showed evidence of H2O2 accumulation. Glyoxylate levels showed no differences between biotypes. Results from this study will contribute to elucidating the mechanism of glyphosate resistance in giant ragweed.
URI: http://hdl.handle.net/10214/8627
Date: 2014-12
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