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Membrane Filtration as a Method of Protein Isolation from the Effluent of a Wheat Wet-Milling Process

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Title: Membrane Filtration as a Method of Protein Isolation from the Effluent of a Wheat Wet-Milling Process
Author: Velicogna, Ronald
Department: Department of Food Science
Program: Food Science
Advisor: Lencki, RobertBock, Jayne
Abstract: The systematic evaluation of ultrafiltration membranes by composition and molecular weight cutoff (MWCO) was performed to evaluate their effectiveness in dewatering effluent from a wheat wet milling process. A polyethersulfone (PES) membrane with the largest MWCO of all the high protein retention membranes evaluated, 100 kDa, was found to retain greater than 99% of the soluble protein. The permeate flux was constant with all membranes tested, demonstrating that the gel layer was the determining factor for permeate flux. A method for semi-quantitatively determining the amount of the three types of alpha amylase inhibitors in wheat wet milling effluent was developed, based on salting out of the proteins followed by SDS-PAGE analysis with a native PAGE verification. Some fractionation of the alpha amylase inhibitor proteins was demonstrated using certain membranes under standard conditions, indicating that alpha amylase inhibitor fractionation and purification processes can potentially be developed using membrane filtration, thus adding value to what is currently a waste effluent stream.
URI: http://hdl.handle.net/10214/8619
Date: 2014-12
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