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Isolation of Salmonella serovar-specific single domain antibodies

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dc.contributor.advisor Hall, J. Christopher
dc.contributor.advisor Tanha, Jamshid
dc.contributor.author Nowacka, Agnieszka
dc.date.accessioned 2014-09-30T17:39:53Z
dc.date.available 2014-09-30T17:39:53Z
dc.date.copyright 2014-09
dc.date.created 2014-09-22
dc.date.issued 2014-09-30
dc.identifier.uri http://hdl.handle.net/10214/8503
dc.description.abstract Worldwide, the human consumption of Salmonella-contaminated food is extremely prevalent. Three of the most common Salmonella serovars known to be involved in infection are Salmonella enterica serovar Typhimurium, Salmonella enterica serovar Enteritidis, and Salmonella enterica serovar Newport. In this proof of concept study, recombinant and serovar-specific Salmonella spp. flagellin and domain 3 (D3) protein antigens were designed and purified. An immune phage display library was established and VHH single domain antibodies (sdAbs) specific for the D3 region of flagellin of Salmonella enterica serovar Typhimurium were isolated. Surface plasmon resonance (SPR) analysis demonstrated that the isolated VHHs possessed nanomolar affinities and were non-cross-reactive to other Salmonella serovars. This work provides a basis for the isolation of serovar-specific VHH sdAbs and the potential future development of an immuno-paper dipstick system, which can be used for detection purposes to help reduce the spread of Salmonella infection. en_US
dc.description.sponsorship Sentinel Bioactive Paper Network, Dr. J. Christopher Hall en_US
dc.language.iso en en_US
dc.subject Salmonella Typhimurium en_US
dc.subject Salmonella Newport en_US
dc.subject Salmonella Enteritidis en_US
dc.subject flagellin en_US
dc.subject flagella en_US
dc.subject Domain 3 en_US
dc.subject D3 en_US
dc.subject VHH en_US
dc.subject single domain antibodies en_US
dc.subject single-domain antibodies en_US
dc.subject Salmonella en_US
dc.subject Salmonellosis en_US
dc.subject detection en_US
dc.subject serovar-specific en_US
dc.subject serovar specificity en_US
dc.subject immunoassay en_US
dc.subject dipstick en_US
dc.subject biosensor en_US
dc.title Isolation of Salmonella serovar-specific single domain antibodies en_US
dc.type Thesis en_US
dc.degree.programme Environmental Sciences en_US
dc.degree.name Master of Science en_US
dc.degree.department Department of Environmental Biology en_US
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