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An Investigation into Plastid Localized Lipases at Membrane Contact Sites with the Endoplasmic Reticulum using Live-Imaging

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dc.contributor.advisor Mathur, Jaideep
dc.contributor.author Mammone, Alena
dc.date.accessioned 2014-09-29T18:26:31Z
dc.date.available 2014-09-29T18:26:31Z
dc.date.copyright 2014-09
dc.date.created 2014-09-24
dc.date.issued 2014-09-29
dc.identifier.uri http://hdl.handle.net/10214/8502
dc.description.abstract Fluorescent protein-based imaging revealed that transient tubules extend and retract from plastids. One hypothesis is that tubulation is a result of pulling force from the endoplasmic reticulum (ER) exerted at membrane contact sites (MCSs). A Brassica napus chloroplast lipase protein 1 (BnCLIP) appeared to mark ER-plastid MCSs; therefore plastids, ER and BnCLIP were observed in live transgenic plants using confocal microscopy. This study found that BnCLIP marked sites where the ER and plastids maintained prolonged contact and where plastid tubules extended by apparent pulling force from the ER. BnCLIP frequency increased significantly following phosphate starvation but not 40mM sucrose treatment. The potential for native Arabidopsis thaliana lipase proteins to localize in a similar manner was explored. This study provides the first demonstration of the role that plastid-ER MCSs play in living cells to modulate the shape and dynamic behaviour of plastids. en_US
dc.description.sponsorship Ontario Graduate Fellowship Program, University of Guelph, Natural Science and Engineering Research Council of Canada, Canada Foundation for Innovation en_US
dc.language.iso en en_US
dc.subject Stromule en_US
dc.subject Lipase en_US
dc.subject Membrane Contact Site en_US
dc.subject Endoplasmic Reticulum en_US
dc.subject Live-Imaging en_US
dc.subject Organelle Extensions en_US
dc.title An Investigation into Plastid Localized Lipases at Membrane Contact Sites with the Endoplasmic Reticulum using Live-Imaging en_US
dc.type Thesis en_US
dc.degree.programme Molecular and Cellular Biology en_US
dc.degree.name Master of Science en_US
dc.degree.department Department of Molecular and Cellular Biology en_US
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