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ISOLATION AND CHARACTERIZATION OF BACTERIOPHAGES AGAINST E.COLI O104:H4 AND PSEUDOMONAS SPP. FOR FOOD BIOCONTROL APPLICATIONS

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dc.contributor.advisor Griffiths, Mansel W
dc.contributor.author Hakeem, Mohammed
dc.date.accessioned 2014-09-23T17:16:31Z
dc.date.available 2014-09-23T17:16:31Z
dc.date.copyright 2014-09
dc.date.created 2014-09-12
dc.date.issued 2014-09-23
dc.identifier.uri http://hdl.handle.net/10214/8487
dc.description Two groups of bacteriophages (bacterial viruses) were studied and used for the biocontrol of two food-related microorganisms. en_US
dc.description.abstract The aim of this study was to determine the effect of isolated and characterized bacteriophages against E. coli O104:H4 and Pseudomonas spp. in food matrices. Ten lytic bacteriophages were isolated against each target microorganism. Only three phages from each group have been selected for characterization and biocontrol studies. Electron microscopy showed that some phages had similar shapes. However, their restriction endonuclease patterns revealed that each phage is unique. The phages were stable at different temperatures and most pH levels. No evidence of the development of resistance in host bacteria was observed when phages were mixed in phage cocktails. The potential of the phage cocktails to control their host in artificial media and real food systems was examined. The phage cocktails of E. coli and Pseudomonas successfully reduced more than 96% of their host population in alfalfa sprouts during germination and fresh salmon, respectively. In contrast, Pseudomonas phage cocktail was unable to control the growth of Pseudomonas spp. in skim milk at 8 °C. en_US
dc.description.sponsorship Sentinel bioactive paper network and Kink Saud University. en_US
dc.language.iso en en_US
dc.subject Foodborne pathogens en_US
dc.subject spoilage en_US
dc.subject E. coli O104:H4 en_US
dc.subject Pseudomonas en_US
dc.subject alfalfa sprouts en_US
dc.subject skim milk en_US
dc.subject and fish en_US
dc.title ISOLATION AND CHARACTERIZATION OF BACTERIOPHAGES AGAINST E.COLI O104:H4 AND PSEUDOMONAS SPP. FOR FOOD BIOCONTROL APPLICATIONS en_US
dc.type Thesis en_US
dc.degree.programme Food Science en_US
dc.degree.name Master of Science en_US
dc.degree.department Department of Food Science en_US
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