Main content

ISOLATION AND CHARACTERIZATION OF BACTERIOPHAGES AGAINST E.COLI O104:H4 AND PSEUDOMONAS SPP. FOR FOOD BIOCONTROL APPLICATIONS

Show full item record

Title: ISOLATION AND CHARACTERIZATION OF BACTERIOPHAGES AGAINST E.COLI O104:H4 AND PSEUDOMONAS SPP. FOR FOOD BIOCONTROL APPLICATIONS
Author: Hakeem, Mohammed
Department: Department of Food Science
Program: Food Science
Advisor: Griffiths, Mansel W
Abstract: The aim of this study was to determine the effect of isolated and characterized bacteriophages against E. coli O104:H4 and Pseudomonas spp. in food matrices. Ten lytic bacteriophages were isolated against each target microorganism. Only three phages from each group have been selected for characterization and biocontrol studies. Electron microscopy showed that some phages had similar shapes. However, their restriction endonuclease patterns revealed that each phage is unique. The phages were stable at different temperatures and most pH levels. No evidence of the development of resistance in host bacteria was observed when phages were mixed in phage cocktails. The potential of the phage cocktails to control their host in artificial media and real food systems was examined. The phage cocktails of E. coli and Pseudomonas successfully reduced more than 96% of their host population in alfalfa sprouts during germination and fresh salmon, respectively. In contrast, Pseudomonas phage cocktail was unable to control the growth of Pseudomonas spp. in skim milk at 8 °C.
Description: Two groups of bacteriophages (bacterial viruses) were studied and used for the biocontrol of two food-related microorganisms.
URI: http://hdl.handle.net/10214/8487
Date: 2014-09
Terms of Use: All items in the Atrium are protected by copyright with all rights reserved unless otherwise indicated.


Files in this item

Files Size Format View Description
Hakeem_Mohammed_201409_MSc.pdf 15.56Mb PDF View/Open Thesis

This item appears in the following Collection(s)

Show full item record