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Zebrafish as a tool to assess Pseudomonas aeruginosa microcolony formation in vivo

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dc.contributor.advisor Khursigara, Cezar
dc.contributor.author Rocker, Adam
dc.date.accessioned 2014-09-19T15:01:50Z
dc.date.available 2014-09-19T15:01:50Z
dc.date.copyright 2014-09
dc.date.created 2014-09-17
dc.date.issued 2014-09-19
dc.identifier.uri http://hdl.handle.net/10214/8477
dc.description.abstract P. aeruginosa is commonly associated with infections in individuals with compromised host-defenses. Critical to persistence within the hostile host environment is the ability to form a biofilm, consisting of a microbial community enmeshed in extracellular polymeric substances. Much of our understanding regarding biofilm has been acquired via confocal microscopic monitoring of flow cell cultures. However, the relevance of these in vitro studies to the complex host environment remains uncertain due to the lack of a vertebral animal model amenable to such detailed visual analyses. This thesis describes the development of a larval zebrafish hindbrain ventricle infection model for the analysis of biofilm formation in vivo. Infection with wild-type P. aeruginosa PAO1 was compared with that of a psl mutant (Δpsl promoter), incapable of producing the critical biofilm exopolysaccharide Psl. Δpsl PAO1 was found to be less fatal, to produce fewer and smaller microcolonies, and to persist poorly throughout infection in comparison to the wild type. The unprecedented microcolony quantification achieved herein is dependent on the optical transparency of the zebrafish, thus demonstrating the power of the zebrafish as a tool for in vivo microcolony analyses. en_US
dc.language.iso en en_US
dc.subject microcolony en_US
dc.subject biofilm en_US
dc.subject pseudomonas aeruginosa en_US
dc.subject PAO1 en_US
dc.subject zebrafish en_US
dc.subject exopolysaccharide en_US
dc.subject psl en_US
dc.title Zebrafish as a tool to assess Pseudomonas aeruginosa microcolony formation in vivo en_US
dc.type Thesis en_US
dc.degree.programme Molecular and Cellular Biology en_US
dc.degree.name Master of Science en_US
dc.degree.department Department of Molecular and Cellular Biology en_US
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