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Understanding the Dlx-mediated effects on cell proliferation

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dc.contributor.advisor Bendall, Andrew
dc.contributor.author MacKenzie, Rachel Kathleen
dc.date.accessioned 2014-09-04T15:24:09Z
dc.date.available 2014-09-04T15:24:09Z
dc.date.copyright 2014-08
dc.date.created 2014-08-21
dc.date.issued 2014-09-04
dc.identifier.uri http://hdl.handle.net/10214/8411
dc.description.abstract Dlx5 and Dlx6 are members of the Distal-less homeobox (Dlx) gene family and encode transcription factors essential for craniofacial patterning and differentiation of mineralizing cell types in the skeleton during development. Forced expression of Dlx5 or Dlx6 in progenitor cells promotes their differentiation and suppresses proliferation in vitro and in vivo. Conversely, studies involving cancer cell lines have shown that Dlx5 can promote proliferation, perhaps via direct transcriptional activation of cell cycle regulatory genes like c-Myc. We have shown that Dlx5 and Dlx6 inhibit cell growth in multipotent progenitor cells and primary limb bud cells and modestly upregulate c-Myc reporter transcription in such cells. Cumulative EdU-incorporation assays indicate that Dlx5 and Dlx6 decrease the proportion of actively cycling cells in a population and may increase the length of the cell cycle. Flow cytometry results suggest a higher proportion of cells in G1 compared to controls. en_US
dc.description.sponsorship Natural Sciences and Engineering Research Council CGS-M en_US
dc.language.iso en en_US
dc.subject Dlx en_US
dc.subject Homeobox en_US
dc.subject Development en_US
dc.subject Cancer en_US
dc.subject Differentiation en_US
dc.subject Proliferation en_US
dc.subject Cell cycle en_US
dc.title Understanding the Dlx-mediated effects on cell proliferation en_US
dc.type Thesis en_US
dc.degree.programme Molecular and Cellular Biology en_US
dc.degree.name Master of Science en_US
dc.degree.department Department of Molecular and Cellular Biology en_US
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