Main content

Investigating the Utility of Next Generation Sequencing for Evaluating Biodiversity in Benthic Communities

Show full item record

Title: Investigating the Utility of Next Generation Sequencing for Evaluating Biodiversity in Benthic Communities
Author: Spall, Jennifer
Department: Department of Integrative Biology
Program: Integrative Biology
Advisor: Hajibabaei, Mehrdad
Abstract: This thesis is an investigation into the current state of two methods of DNA sequencing and their ability to assess the biodiversity of benthic samples containing the taxa generally used in biomonitoring studies. Its goal was to evaluate the ability of DNA Barcoding through Sanger sequencing and Next Generation Sequencing through 454 pyrosequencing to provide biodiversity information that would prove useful to biomonitoring organisations such as the Toronto and Region Conservation Authority. The investigation was performed by collecting benthic samples at four locations in the Humber River and determining the biodiversity captured by each sequencing technique. Further analyses were performed to determine the extent to which the known drawbacks of 454 pyrosequencing affect its ability to be useful in this regard. It was found that in the absence of primer binding biases the two sequencing techniques are statistically equal in their ability to measure the biodiversity of benthic samples.
URI: http://hdl.handle.net/10214/8382
Date: 2014-09


Files in this item

Files Size Format View
Spall_Jennifer_201408_MSc.pdf 2.613Mb PDF View/Open

This item appears in the following Collection(s)

Show full item record