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Programmed Death Ligand 2 Gene Regulation in T-cells During Homeostasis and Lentiviral Infection

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Title: Programmed Death Ligand 2 Gene Regulation in T-cells During Homeostasis and Lentiviral Infection
Author: MacMillan, Beth
Department: Department of Pathobiology
Program: Pathobiology
Advisor: Bienzle, Dorothee
Abstract: Programmed Death Ligand 2 (PD-L2) is a co-stimulatory molecule predominantly expressed on antigen-presenting cells. PD-L2 interacts with membrane-bound receptor PD-1 on activated T-cells. This interaction transmits a negative signal to T-cells inducing anergy or apoptosis. Viruses such as HIV induce PD-L2, which enables viral persistence. In this study, regulation of PD-L2 gene expression in feline lymphocytes in homeostasis and lentiviral infection was investigated. The upstream region of PD-L2 was amplified and sequenced to identify putative transcription factor binding sites, including NF-κB, STAT6, Oct-1/2, GATA-3 and NFAT. Assays to assess transcriptional activation from incremental segments upstream of PD-L2 identified the first 2 kb as the possible location of the proximal promoter. Transfection of NF-κB or NFAT into T-cells increased PD-L2 transcription, but FIV infection did not. Results suggest that transcriptional activation of PD-L2 in homeostatic T-cells differs from macrophages and DCs; and that acute FIV infection does not affect PD-L2 expression.
URI: http://hdl.handle.net/10214/8335
Date: 2014-08
Rights: Attribution-NonCommercial-NoDerivs 2.5 Canada


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Attribution-NonCommercial-NoDerivs 2.5 Canada Except where otherwise noted, this item's license is described as Attribution-NonCommercial-NoDerivs 2.5 Canada