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Palmitoyl-CoA Inhibition of Mitochondrial ADP Sensitivity is Attenuated by Exercise Training in Human Skeletal Muscle

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dc.contributor.advisor Holloway, Graham
dc.contributor.author Ludzki, Alison
dc.date.accessioned 2014-08-14T19:00:18Z
dc.date.available 2014-08-14T19:00:18Z
dc.date.copyright 2014-08
dc.date.created 2014-08-07
dc.date.issued 2014-08-14
dc.identifier.uri http://hdl.handle.net/10214/8287
dc.description.abstract A hallmark of improved metabolic control is a reduced free ADP requirement for a given workload (increased ADP sensitivity). In contrast to in vivo data, in situ assessments suggest that mitochondrial ADP sensitivity is decreased following exercise training, implying external regulation that is not recapitulated in situ. One previously unexplored regulator is palmitoyl-CoA (P-CoA), a lipid metabolism intermediate that inhibits the mitochondrial ADP transport protein adenine nucleotide transferase (ANT). This thesis: 1) established reduced mitochondrial ADP sensitivity following exercise training in middle aged males using permeabilized muscle fibre bundles (PmFB), 2) determined a methodology to evaluate ADP kinetics in PmFB in the presence of P-CoA, and 3) found increased mitochondrial ADP sensitivity in the presence of P-CoA following training. These data suggest that P-CoA is a key regulator of oxidative phosphorylation and direct future exploration of mitochondrial function towards the control of ADP transport via ANT and the effects of exercise on the P-CoA-ANT interaction en_US
dc.description.sponsorship NSERC, CFI en_US
dc.language.iso en en_US
dc.subject palmitoyl-CoA en_US
dc.subject Mitochondria en_US
dc.subject Exercise en_US
dc.subject Skeletal Muscle en_US
dc.title Palmitoyl-CoA Inhibition of Mitochondrial ADP Sensitivity is Attenuated by Exercise Training in Human Skeletal Muscle en_US
dc.type Thesis en_US
dc.degree.programme Human Health and Nutritional Sciences en_US
dc.degree.name Master of Science en_US
dc.degree.department Department of Human Health and Nutritional Sciences en_US
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