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Identification and characterization of genes involved in the fruit color development of European plums (Prunus domestica L.)

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Title: Identification and characterization of genes involved in the fruit color development of European plums (Prunus domestica L.)
Author: Selvaraj, Dinesh Kumar
Department: Department of Plant Agriculture
Program: Plant Agriculture
Advisor: Subramanian, Jayasankar
Abstract: European plum fruits (Prunus domestica L.) are normally blue-black. However, some genotypes are not blue-black and remain green/yellow after ripening. We hypothesize that in such genotypes anthocyanin biosynthesis is genetically affected. In this study, six European plum genotypes with diverse colors are investigated for expression of anthocyanin biosynthetic pathway genes - phenylalanine ammonia lyase (PAL), chalcone synthase (CHS), dihydroflavonol 4-reductase (DFR), anthocyanin synthase (ANS), UDP-glucose:flavonoid 3-O-glucosyltransferase 1 (UFGT 1) and UDP-glucose:flavonoid 3-O-glucosyl transferase 2 (UFGT 2). Further total polyphenols and individual flavonoid compounds were also estimated. To determine the function, Pd-CHS, Pd-DFR, Pd-ANS, Pd-UFGT were overexpressed in tobacco. Our results show that the expression levels of Pd-CHS and Pd-UFGT 2 were downregulated in off-color genotypes. Tobacco plants expressing Pd-CHS showed an increase in petal color intensity compared to control. These results suggest that CHS may be the rate limiting step in anthocyanin production of within off-color genotypes of European plums. Key words: plums, anthocyanins, expression, overexpression, transgenic tobacco
URI: http://hdl.handle.net/10214/8219
Date: 2014-06
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