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Influence of Novel Maize Starches on Human Colonic Microbial Fermentation

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Title: Influence of Novel Maize Starches on Human Colonic Microbial Fermentation
Author: Brown, Ian Harrison Lee
Department: Department of Molecular and Cellular Biology
Program: Molecular and Cellular Biology
Advisor: Allen-Vercoe, Emma
Abstract: This study evaluated differences between in vitro, small-scale batch fermentations of 6 different maize starch substrates by human gut microbiota. Stable fecal communities from 3 donors, grown in chemostats modeling the distal colon, were used as fecal inocula in batch fermentations. Denaturing gradient gel electrophoresis (DGGE) and gas chromatography-mass spectrometry (GC-MS) were used to assess changes in community structure and production of metabolites such as short chain fatty acids (SCFAs) for each fecal community. The tested starch substrates promoted unique changes to the fecal communities of each donor, suggesting that an individual's fecal microbiota ferments various starch substrates in different ways. GC-MS data support these donor specific results, indicating a significant production of butyrate for all 3 donor's microbiota, while significant increases in pentanoic and propanoic acid were observed in just one. Although community profiles exhibited changes according to starch substrate fermented, no clear differences in metabolites were observed.
URI: http://hdl.handle.net/10214/8212
Date: 2014


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