dc.contributor.advisor |
Merrill, Rod |
|
dc.contributor.author |
Krska, Daniel
|
|
dc.date.accessioned |
2014-05-12T16:43:43Z |
|
dc.date.available |
2014-05-12T16:43:43Z |
|
dc.date.copyright |
2014-04 |
|
dc.date.created |
2014-04-14 |
|
dc.date.issued |
2014-05-12 |
|
dc.identifier.uri |
http://hdl.handle.net/10214/8117 |
|
dc.description.abstract |
Mono-ADP-ribosyltransferase (mART) toxins are a class of bacterial proteins which function by cleaving the nicotinamide moiety from NAD+ and attaching the ADP-ribose to a host protein, to alter its function. These are important virulence factors for many diseases. Larvin is a novel mART toxin from Paenibacillus larvae, the causative agent of American Foulbrood in honey bees. Larvin targets RhoA as a substrate for its transferase reaction, and kinetics for the RhoA substrate were characterized for the first time for the mART C3 subgroup. A small molecule inhibitor of Larvin enzymatic activity was discovered, the first known inhibitor for C3 toxins. Larvin was crystallized, and its crystal structure was solved to 2.3 Å resolution. Larvin was also shown to have a different mechanism of cell entry from other C3 toxins. Characterization of Larvin allows for a fuller understanding of the mART family, and for development of better inhibitors as potential therapeutics. |
en_US |
dc.language.iso |
en |
en_US |
dc.rights |
Attribution-NonCommercial-NoDerivs 2.5 Canada |
* |
dc.rights.uri |
http://creativecommons.org/licenses/by-nc-nd/2.5/ca/ |
* |
dc.subject |
Biochemistry |
en_US |
dc.subject |
Enzyme Kinetics |
en_US |
dc.subject |
mono-ADP-ribostyltransferase |
en_US |
dc.subject |
Protein Structure |
en_US |
dc.subject |
Protein Toxin |
en_US |
dc.subject |
Enzyme Inhibition |
en_US |
dc.subject |
Fluorescence Assay |
en_US |
dc.title |
Larvin: The characterization of a novel mono-ADP-ribosyltransferase toxin |
en_US |
dc.type |
Thesis |
en_US |
dc.degree.programme |
Biophysics |
en_US |
dc.degree.name |
Master of Science |
en_US |
dc.degree.department |
Department of Molecular and Cellular Biology |
en_US |
dc.rights.license |
All items in the Atrium are protected by copyright with all rights reserved unless otherwise indicated. |
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