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Investigating probiotic biofilms: probing the link between biofilm formation and antimicrobial production

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dc.contributor.advisor Khursigara, Cezar
dc.contributor.author Vis, Emily
dc.date.accessioned 2014-05-07T13:48:00Z
dc.date.available 2014-05-07T13:48:00Z
dc.date.copyright 2014-04
dc.date.created 2014-04-16
dc.date.issued 2014-05-07
dc.identifier.uri http://hdl.handle.net/10214/8083
dc.description.abstract Probiotics are live microorganisms known to confer a host benefit when ingested and represent a feasible option to alleviate gastrointestinal microbiota imbalances. Alleviation may occur via several mechanisms including bacteriocin production by probiotics to limit pathogen colonization. Unfortunately, probiotic efficacy may be restricted by their inability to incorporate into the naturally- occurring, static biofilms lining the human gut. This thesis examines the biofilm forming ability of fourteen commercial probiotic strains via a high-throughput crystal violet assay and scanning electron microscopy. All probiotics formed biofilms in vitro in at least one condition tested. More specifically, strong biofilm formation was observed by Pediococcus acidilactici under several conditions. Further investigation demonstrated that both anaerobiosis and bacteriocin production alter P. acidilactici biofilm formation. Moreover, it was shown that aeration, medium, and mode of growth modify bacteriocin production from P. acidilactici, highlighting the importance of environmental factors that influence both biofilm formation and bacteriocin production. en_US
dc.description.sponsorship OMAFRA en_US
dc.language.iso en en_US
dc.rights Attribution 2.5 Canada *
dc.rights.uri http://creativecommons.org/licenses/by/2.5/ca/ *
dc.subject probiotic en_US
dc.subject biofilm en_US
dc.subject Pediococcus acidilactici en_US
dc.subject pediocin en_US
dc.title Investigating probiotic biofilms: probing the link between biofilm formation and antimicrobial production en_US
dc.type Thesis en_US
dc.degree.programme Molecular and Cellular Biology en_US
dc.degree.name Master of Science en_US
dc.degree.department Department of Molecular and Cellular Biology en_US
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Attribution 2.5 Canada Except where otherwise noted, this item's license is described as Attribution 2.5 Canada