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Developing Methodologies for the Investigation of Free-living Amoeba as a Tool for Pathogen Surveillance on Dairy Farms and Aquaculture

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dc.contributor.advisor Lucy, Mutharia McLean, John 2014-05-06T18:41:50Z 2014-05-06T18:41:50Z 2014-04 2014-04-25 2014-05-06
dc.description.abstract Free-living amoeba are phagocytic protozoans that act as environmental reservoirs, a protective niche, and a vehicle for transmission for amoeba-resistant bacterial pathogens. Many amoeba-resistant bacteria have been identified using only laboratory-adapted Acanthamoeba. We isolated resident amoeba from target environments of dairy farms and aquaculture settings to evaluate their use as a pathogen detection tool. Amoeba were only isolated from 3 of 23 (13%) environmental samples using established methods. A two-step sample decontamination protocol was developed and led to the isolation of 14 additional amoeba. An amoeba co-culture method was developed to assess the survival of 12 mycobacterial species within environmental and laboratory-adapted amoeba. Major strain differences were observed at the amoeba level which had drastic effects on the survival of different bacterial species within individual amoeba. Targeted isolation of resident bacteria from soils and feces using amoebal enrichment protocols were unsuccessful. However, the methodologies developed in this study provide a valid technical starting point for future studies. en_US
dc.description.sponsorship Ontario Ministry of the Environment, Ontario Ministry of Agriculture, Food and Rural Affairs en_US
dc.language.iso en en_US
dc.publisher University of Guelph en_US
dc.rights Attribution-NoDerivs 2.5 Canada *
dc.rights.uri *
dc.subject Free-living amoeba en_US
dc.subject Environmental reservoirs en_US
dc.subject Dairy farms en_US
dc.subject Aquaculture en_US
dc.subject Phagocytic protozoans en_US
dc.title Developing Methodologies for the Investigation of Free-living Amoeba as a Tool for Pathogen Surveillance on Dairy Farms and Aquaculture en_US
dc.type Thesis en_US Molecular and Cellular Biology en_US Master of Science en_US Department of Molecular and Cellular Biology en_US University of Guelph en_US

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Attribution-NoDerivs 2.5 Canada Except where otherwise noted, this item's license is described as Attribution-NoDerivs 2.5 Canada
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