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Developing Methodologies for the Investigation of Free-living Amoeba as a Tool for Pathogen Surveillance on Dairy Farms and Aquaculture

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dc.contributor.advisor Lucy, Mutharia
dc.contributor.author McLean, John
dc.date.accessioned 2014-05-06T18:41:50Z
dc.date.available 2014-05-06T18:41:50Z
dc.date.copyright 2014-04
dc.date.created 2014-04-25
dc.date.issued 2014-05-06
dc.identifier.uri http://hdl.handle.net/10214/8072
dc.description.abstract Free-living amoeba are phagocytic protozoans that act as environmental reservoirs, a protective niche, and a vehicle for transmission for amoeba-resistant bacterial pathogens. Many amoeba-resistant bacteria have been identified using only laboratory-adapted Acanthamoeba. We isolated resident amoeba from target environments of dairy farms and aquaculture settings to evaluate their use as a pathogen detection tool. Amoeba were only isolated from 3 of 23 (13%) environmental samples using established methods. A two-step sample decontamination protocol was developed and led to the isolation of 14 additional amoeba. An amoeba co-culture method was developed to assess the survival of 12 mycobacterial species within environmental and laboratory-adapted amoeba. Major strain differences were observed at the amoeba level which had drastic effects on the survival of different bacterial species within individual amoeba. Targeted isolation of resident bacteria from soils and feces using amoebal enrichment protocols were unsuccessful. However, the methodologies developed in this study provide a valid technical starting point for future studies. en_US
dc.description.sponsorship Ontario Ministry of the Environment, Ontario Ministry of Agriculture, Food and Rural Affairs en_US
dc.language.iso en en_US
dc.rights Attribution-NoDerivs 2.5 Canada *
dc.rights.uri http://creativecommons.org/licenses/by-nd/2.5/ca/ *
dc.title Developing Methodologies for the Investigation of Free-living Amoeba as a Tool for Pathogen Surveillance on Dairy Farms and Aquaculture en_US
dc.type Thesis en_US
dc.degree.programme Molecular and Cellular Biology en_US
dc.degree.name Master of Science en_US
dc.degree.department Department of Molecular and Cellular Biology en_US


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Attribution-NoDerivs 2.5 Canada Except where otherwise noted, this item's license is described as Attribution-NoDerivs 2.5 Canada