Main content

Developing Methodologies for the Investigation of Free-living Amoeba as a Tool for Pathogen Surveillance on Dairy Farms and Aquaculture

Show full item record

Title: Developing Methodologies for the Investigation of Free-living Amoeba as a Tool for Pathogen Surveillance on Dairy Farms and Aquaculture
Author: McLean, John
Department: Department of Molecular and Cellular Biology
Program: Molecular and Cellular Biology
Advisor: Lucy, Mutharia
Abstract: Free-living amoeba are phagocytic protozoans that act as environmental reservoirs, a protective niche, and a vehicle for transmission for amoeba-resistant bacterial pathogens. Many amoeba-resistant bacteria have been identified using only laboratory-adapted Acanthamoeba. We isolated resident amoeba from target environments of dairy farms and aquaculture settings to evaluate their use as a pathogen detection tool. Amoeba were only isolated from 3 of 23 (13%) environmental samples using established methods. A two-step sample decontamination protocol was developed and led to the isolation of 14 additional amoeba. An amoeba co-culture method was developed to assess the survival of 12 mycobacterial species within environmental and laboratory-adapted amoeba. Major strain differences were observed at the amoeba level which had drastic effects on the survival of different bacterial species within individual amoeba. Targeted isolation of resident bacteria from soils and feces using amoebal enrichment protocols were unsuccessful. However, the methodologies developed in this study provide a valid technical starting point for future studies.
URI: http://hdl.handle.net/10214/8072
Date: 2014-04
Rights: Attribution-NoDerivs 2.5 Canada


Files in this item

Files Size Format View
McLean_John_201404_MSc.pdf 3.178Mb PDF View/Open

This item appears in the following Collection(s)

Show full item record

Attribution-NoDerivs 2.5 Canada Except where otherwise noted, this item's license is described as Attribution-NoDerivs 2.5 Canada
The library is committed to ensuring that members of our user community with disabilities have equal access to our services and resources and that their dignity and independence is always respected. If you encounter a barrier and/or need an alternate format, please fill out our Library Print and Multimedia Alternate-Format Request Form. Contact us if you’d like to provide feedback: lib.a11y@uoguelph.ca  (email address)