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Successful Cryopreservation of Asian elephant (Elephas maximus) semen using simple techniques

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Title: Successful Cryopreservation of Asian elephant (Elephas maximus) semen using simple techniques
Author: Arnold, Danielle
Department: Department of Animal and Poultry Science
Program: Animal and Poultry Science
Advisor: Bédécarrats, Gregoy
Abstract: This thesis is an investigation of the effect of simple freezing techniques, storage at 4°C and different thaw temperatures on post-thaw measures of cryopreserved Asian elephant semen samples. There was a significant effect of freeze rate on post-thaw motility (P<0.0001) and acrosomal integrity (P<0.005) of straws frozen after 0 and 24 hr storage at 4°C. The faster freeze rates in the dry shipper and at 1cm or 2 cm above liquid nitrogen consistently provided better cryopreservation than slower freezing rates. Thaw temperature had no effect on post-thaw semen quality. Storage of samples prior to freezing had a detrimental effect on post-thaw semen quality. Placing straws directly in a dry shipper to freeze and thawing them at 50°C was a simple technique for effectively cryopreserving Asian elephant semen.
Date: 2014-04
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